A herbicide-resistance-based rape hybrid seed producing method and applications thereof
A herbicide and hybrid technology, applied in the field of rape hybrid seed production, can solve the problems of high requirement of cytoplasmic male sterility material, negative effect of sterile cytoplasmic yield, difficulty in identifying and removing impurities, etc., to ensure the purity and commerciality of hybrids. , the effect of increasing genetic diversity and reducing labor costs
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[0074] Below in conjunction with accompanying drawing and embodiment describe in detail:
[0075] 1. Method
[0076] 1.1. This method is as follows: figure 1 shown.
[0077] 1.1.1. Introduce the broad-spectrum herbicide resistance gene into the rapeseed parent line to obtain the herbicide-resistant rapeseed line; obtain the herbicide-resistant rapeseed line through hybridization, backcrossing, or / and microspore culture. Herbicide lines are used as male parents for hybrid seed production: e.g. figure 2 ,
[0078] In the first year (season), the herbicide-resistant variety (code B, such as 12WH318 or Z7B10) was used as the herbicide-resistant donor parent to cross with the excellent recipient parent A to obtain F 1 generation;
[0079] In the second year (season), parent A and F above 1 BC 1 Generation, spraying corresponding herbicides to screen out excellent herbicide-resistant individual plants;
[0080] Year 3 (season), with herbicide-resistant BC 1 BC 2 Groups, s...
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