Primer and kit for detecting dominant hereditary cerebral artery disease related gene and application

A kit and gene technology, applied in DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of undiagnosed patients, and achieve the effect of fewer types, simple operation, and easy application.

Inactive Publication Date: 2016-11-23
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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Problems solved by technology

[0005] In clinical cases, there are still patients who cannot be diagnosed based on the above techniques

Method used

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  • Primer and kit for detecting dominant hereditary cerebral artery disease related gene and application
  • Primer and kit for detecting dominant hereditary cerebral artery disease related gene and application
  • Primer and kit for detecting dominant hereditary cerebral artery disease related gene and application

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Embodiment Construction

[0019] The technical solution of the present invention is described in detail below. The embodiments of the present invention are only used to illustrate the technical solutions of the present invention without limitation. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the invention can be modified or equivalently replaced , without departing from the spirit and scope of the technical solution of the present invention, all of which shall be covered by the claims of the present invention.

[0020] The present invention relates to molecular biology experiment, if not specially noted, can refer to from " molecular cloning " book (J. Sambrook, E.F. Fritsch, T. Mani Atis works, Science Press, 1994) . This book and its subsequent publications are the most commonly used guiding reference books for those skilled in the art when performing experimental o...

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Abstract

The invention provides a primer pair, a primer and kit for detecting a dominant hereditary cerebral artery disease related gene and application. The primer pair consists of a forward primer provided with a sequence shown by SEQIDNo.1 or its sequence and a reverse primer provided with a sequence shown in SEQIDNo.2 or its homologous sequence. The primer is applicable to detection of autosomal dominant genetic disease cerebral arteries (CADASIL) related genes of subcortical infarcts and leukoencephalopathy and judgment whether the p.C201Y loci of a NOTCH3 gene mutates or not, and a reference is provided for clinical diagnosis.

Description

technical field [0001] The invention relates to a detection technology of disease-related genes, in particular to a primer for detecting CADASIL-related genes, a detection kit and its application. Background technique [0002] Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leucoencephalopathy (CADASIL) is a relatively rare small artery disease that occurs in adults and is inherited in a dominant manner Vascular disease. Patients often have cerebral infarction or transient ischemic attack caused by cerebrovascular occlusion, dementia, migraine with aura, and mental problems (Chinese Journal of Stroke, 2010, 5(7), 573-8). [0003] Studies have found that the mutation of the NOTCH3 gene located in the region of chromosome 19p13.1-13.2 is the pathogenic cause of the disease. NOTCH3 has 33 exons, and the mutations related to pathogenicity are concentrated in exons 2-24. In the Asian population, the mutation rate of exon 4 is the highest, accounting for ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 苏敬敬刘建仁翟宇陶晓晓
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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