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Method for preparing diglyceride through lard glycerolysis catalysis by power ultrasonic pretreatment enzyme method

A technology of ultrasonic pretreatment and diglyceride is applied in the field of preparing diglyceride, and power ultrasonic pretreatment enzyme catalyzes the hydrolysis of lard glycerol to prepare diglyceride, so as to improve the speed and yield, increase the value of by-products, and reduce environmental pollution Effect

Inactive Publication Date: 2016-11-23
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have shown that ultrasound-assisted enzymatic reactions can increase the rate and yield of the reaction, but there are few reports on the use of ultrasonic pretreatment enzymes to catalyze the synthesis of diacylglycerol

Method used

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  • Method for preparing diglyceride through lard glycerolysis catalysis by power ultrasonic pretreatment enzyme method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Take a certain amount of lard and put it in a water bath to completely melt it, then mix it with glycerin in a conical flask according to 1:1 (molar ratio);

[0021] 2. Add 4% lard weight of immobilized lipase Lipozyme RMIM to the mixture of lard and glycerin, place the Erlenmeyer flask in a constant temperature water bath, insert the ultrasonic probe about 5 mm below the reaction solution, and ultrasonically Under the conditions of temperature and ultrasonic power of 200 W, ultrasonic treatment was performed at a certain stirring speed for 5 min, transferred to a constant temperature oscillator in a water bath, and reacted at 50 °C and a stirring speed of 150 r / min for 4 h;

[0022] Three, filter and remove lipase from above-mentioned reaction mixture, promptly make the fat containing diglyceride;

[0023] Four, utilize thin layer chromatography to carry out qualitative analysis to the composition of gained reaction mixture, utilize normal phase high performance liq...

Embodiment 2

[0025] 1. Take a certain amount of lard and put it in a water bath to completely melt it, then mix it with glycerin in a conical flask according to 1:1 (molar ratio);

[0026] 2. Add 3% lard weight of immobilized lipase Lipozyme RMIM to the mixture of lard and glycerin, place the Erlenmeyer flask in a constant temperature water bath, insert the ultrasonic probe about 5 mm below the reaction solution, and ultrasonically Under the conditions of temperature and ultrasonic power of 200 W, ultrasonic treatment was performed at a certain stirring speed for 5 min, transferred to a constant temperature oscillator in a water bath, and reacted at 50 °C and a stirring speed of 150 r / min for 4 h;

[0027] Three, filter and remove lipase from above-mentioned reaction mixture, promptly make the fat containing diglyceride;

[0028] Four, utilize thin layer chromatography to carry out qualitative analysis to the composition of gained reaction mixture, utilize normal phase high performance liq...

Embodiment 3

[0030] 1. Take a certain amount of lard and put it in a water bath to completely melt it, then mix it with glycerin in a conical flask according to 1:1 (molar ratio);

[0031] 2. Add 3% lard weight of immobilized lipase Lipozyme RMIM to the mixture of lard and glycerin, place the Erlenmeyer flask in a constant temperature water bath, insert the ultrasonic probe about 5 mm below the reaction solution, and ultrasonically Temperature and ultrasonic power of 250 W, ultrasonic treatment at a certain stirring speed for 5 minutes, transferred to a constant temperature oscillator in a water bath, and reacting for 4 hours at 50 °C and a stirring speed of 150 r / min;

[0032] Three, filter and remove lipase from above-mentioned reaction mixture, promptly make the fat containing diglyceride;

[0033] 4. Utilize thin-layer chromatography to qualitatively analyze the composition of the resulting reaction mixture, and utilize normal phase high-performance liquid chromatography to quantitativ...

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Abstract

The invention discloses a method for preparing diglyceride through lard glycerolysis catalysis by a power ultrasound pretreatment enzyme method. The method comprises the steps that 1, a certain quantity of lard is placed in a water bath kettle to be completely melted and is then mixed with glycerinum in a conical flask; 2, immobilized lipase Lipozyme RMIM is added into a lard and glycerinum mixture; then, the mixture is put in a constant temperature water bath for ultrasonic pretreatment, and is then transferred into a water bath constant temperature oscillator for reaction; 3, the lipase is removed from the reaction mixture through filtering, and grease containing diglyceride is prepared. The method has the advantages that the low-cost animal product processing by-product of lard is well utilized; the by-product value is improved; high speed and high efficiency are realized; no solvent residue exists; the product can be easily separated from enzyme; a method for fast and efficiently preparing healthy functional diglyceride is provided.

Description

technical field [0001] The invention belongs to the technical field of lard deep processing, and relates to a method for preparing diglyceride, in particular to a method for preparing diglyceride by power ultrasonic pretreatment enzyme catalyzing lard glycerol hydrolysis in a solvent-free system. Background technique [0002] Lard, commonly known as lard, is a high-level saturated fatty acid glyceride. my country has very rich lard resources. With the change of people's dietary concepts and the improvement of living standards, the consumption of lard is decreasing due to the high cholesterol content of lard, and the phenomenon of "surplus" lard appears. It is of great significance to develop nutritious and healthy lard products. [0003] Diacylglycerol (DAG) is a product of esterification of two hydroxyl groups in glycerol with fatty acids, and is a trace component present in various natural edible oils. After review by the U.S. Food and Drug Administration, DAG oil is reco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 孔保华赵欣欣姜帅殷小玉杜洪振
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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