A kind of anti-cropping fungus agent
A technology of stubble bacteria agent and bacteria residue, applied in the field of anti-continuous bacteria agent, can solve the problems of low number of viable bacteria, large energy consumption, and insignificant effect.
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Embodiment 1
[0106] Embodiment 1: Preparation of the anti-cropping bacterial agent of the present invention
[0107] The implementation steps of this embodiment are as follows:
[0108] First, prepare the Pythium oligandrum ferment
[0109] A. Preparation of CMA medium
[0110] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0111] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0112] B. Activation culture
[0113] 200g of potatoes, 20g of glucose, 15-20g of agar and 1000ml of tap water were used to prepare PDA medium by conventional methods.
[0114] Use a steril...
Embodiment 2
[0124] Embodiment 2: Preparation of the anti-cropping bacterial agent of the present invention
[0125] The implementation steps of this embodiment are as follows:
[0126] First, prepare the Pythium oligandrum ferment
[0127] A. Preparation of CMA medium
[0128] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0129] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Jinjiufeng Technology Development Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0130] B. Activation culture
[0131] PDA medium was prepared in the same manner as in Example 1.
[0132] Use a sterile inoculation loop to pick a small amount of mycelium fro...
Embodiment 3
[0142] Embodiment 3: preparation of the anti-cropping bacterial agent of the present invention
[0143] The implementation steps of this embodiment are as follows:
[0144] First, prepare the Pythium oligandrum ferment
[0145] A. Preparation of CMA medium
[0146] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;
[0147] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;
[0148] B. Activation culture
[0149] PDA medium was prepared in the same manner as in Example 1.
[0150] Use a sterile inoculation loop to pick a small amount of mycelium from the culture...
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