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A kind of anti-cropping fungus agent

A technology of stubble bacteria agent and bacteria residue, applied in the field of anti-continuous bacteria agent, can solve the problems of low number of viable bacteria, large energy consumption, and insignificant effect.

Inactive Publication Date: 2017-06-30
三门峡龙飞生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the bottleneck problem that hinders the agricultural application of anti-cropping inoculum is that the microbial fermentation inoculum obtained by traditional microbial fermentation method has a low number of viable bacteria, single function, and the effect is not obvious when applied in agricultural production.
And in the process of bacterial agent production, the energy consumption is large, and the resource utilization rate is low

Method used

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  • A kind of anti-cropping fungus agent
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  • A kind of anti-cropping fungus agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] Embodiment 1: Preparation of the anti-cropping bacterial agent of the present invention

[0107] The implementation steps of this embodiment are as follows:

[0108] First, prepare the Pythium oligandrum ferment

[0109] A. Preparation of CMA medium

[0110] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;

[0111] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;

[0112] B. Activation culture

[0113] 200g of potatoes, 20g of glucose, 15-20g of agar and 1000ml of tap water were used to prepare PDA medium by conventional methods.

[0114] Use a steril...

Embodiment 2

[0124] Embodiment 2: Preparation of the anti-cropping bacterial agent of the present invention

[0125] The implementation steps of this embodiment are as follows:

[0126] First, prepare the Pythium oligandrum ferment

[0127] A. Preparation of CMA medium

[0128] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;

[0129] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Jinjiufeng Technology Development Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;

[0130] B. Activation culture

[0131] PDA medium was prepared in the same manner as in Example 1.

[0132] Use a sterile inoculation loop to pick a small amount of mycelium fro...

Embodiment 3

[0142] Embodiment 3: preparation of the anti-cropping bacterial agent of the present invention

[0143] The implementation steps of this embodiment are as follows:

[0144] First, prepare the Pythium oligandrum ferment

[0145] A. Preparation of CMA medium

[0146] Boil 60g corn kernels in 1000mL distilled water for 90min, filter with double-layer gauze, add 20g glucose to the obtained filtrate, then add water to make up to 1000mL, subpackage, and sterilize at a temperature of 121°C for 20min to obtain the CMA Culture medium;

[0147] Inoculate 0.1 g of Pythium oligandrum provided by Beijing Biorui Biotechnology Co., Ltd. into 200 mL of CMA liquid medium, and carry out shaking culture in a shaking culture shaker at a temperature of 28 ° C and a rotation speed of 180 r / min for 4 days;

[0148] B. Activation culture

[0149] PDA medium was prepared in the same manner as in Example 1.

[0150] Use a sterile inoculation loop to pick a small amount of mycelium from the culture...

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Abstract

Disclosed is a continuous-cropping bacteria resistant agent. The continuous-cropping bacteria resistant agent is composed of bacillus licheniformis bacteria powder, streptomyces microflavus powder, pythium oligandrum yeasts, green wooden mold powder, edible fungi residues, compound sodium nitrophenolate and alpha-naphthalene acetic acid. By the arrangement, continuous-cropping diseases of crops can be alleviated, diseases of bacterial wilt, tobacco mosaic and the like caused by continuous cropping are highly prevented, various diseases of thielariopsis basicola, blight, root-knot nematode, damping off and the like can be prevented, and incidence rate and disease index of flue-cured tobacco black shank disease, the thielariopsis basicola and common mosaic are effectively reduced; by the aid of the continuous-cropping bacteria resistant agent, flue-cured tobacco can be promoted in early growth and quick tiller, growth period is brought forward, plant height and stem thickness of the flue-cured tobacco are increased, yield of flue-cured tobacco leaves is increased, and appearance quality of the tobacco leaves is improved.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of agricultural microorganisms. More specifically, the present invention relates to an anti-cropping fungus. [0003] 【Background technique】 [0004] With the continuous reform of the planting system, the planting area has become tighter, and the problem of repeated cropping has become one of the difficulties encountered in production. Repeated cropping disease refers to the physiological symptoms that occur when the same crop is planted on the same land year after year. The main cause of double-cropping disease is that the same crop is planted year after year, resulting in the lack or imbalance of nutrient elements preferred by crops in the soil. The root exudates and residues of the previous crops are decomposed to produce toxic substances that cause poisoning and growth inhibition to the crops of the next year. The pathogenic microorganisms in the soil accumulate year by year, which gradually ag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05G3/02C05G3/04C05G3/00C12N1/14C12R1/645C05G3/60C05G3/80
CPCC05B7/00C05D9/00C05G3/00C05G3/60C05G3/80C12N1/14C05F5/00C05F5/002C05F5/006C05F11/08C05F9/04
Inventor 赵跃进赵跃文赵跃民易卿杜亚楠赵洁赵科谢松张盼任寸娟秦中辉赵九生
Owner 三门峡龙飞生物工程有限公司
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