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Multistage nano golden flower, preparation method and application thereof

A nano-gold and nano-technology, which is applied in the field of multi-level structure nano-gold, can solve the problems of poor repeatability, non-obvious multi-level structure, and small specific surface area of ​​nano-gold, so as to achieve the effect of easy capture and improved detection sensitivity

Active Publication Date: 2016-11-23
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the specific surface area of ​​the nano-golden flower prepared by the existing method is relatively small, the multi-level structure is not obvious, the synthesis steps are many, and the reproducibility is poor.

Method used

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  • Multistage nano golden flower, preparation method and application thereof
  • Multistage nano golden flower, preparation method and application thereof
  • Multistage nano golden flower, preparation method and application thereof

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preparation example Construction

[0038] In a more typical embodiment, a method for preparing nano gold flowers may include the following steps:

[0039] 1) Sodium citrate reduces chloroauric acid to prepare nano-gold sphere particles with a particle size of 10 nm to 30 nm, which are used as seeds for preparing nano-gold flower particles.

[0040] 2) Add chloroauric acid into polydiallyldimethylammonium chloride (PDDA) aqueous solution, stir at 25°C for more than 15 minutes, until the solution turns yellow, then quickly add ascorbic acid aqueous solution into the above yellow solution, and stir vigorously For more than 30 seconds, add the aforementioned gold nanosphere seeds, stir rapidly for more than 10 seconds, and stand at room temperature for more than 4 hours.

[0041] Another aspect of the present invention provides the use of the multi-level golden nano particles. For example, it is used as a gold-labeled antibody for immunochromatographic test strips in the fields of biological and chemical detection...

Embodiment 1

[0074] (1) Preparation of nano-golden flower particles with multi-level structure: the synthesis of nano-golden flowers is based on the regrowth method of nano-crystal seeds, and mainly includes the following steps:

[0075] First, the synthesis of gold nanoseed crystals: 100 ml, 2.5×10 -4 mol / L aqueous solution of chloroauric acid is heated to 120 ℃ , add 10 milliliters, 1% sodium citrate aqueous solution, after stirring for 30 minutes, the solution turns wine red, stop heating and cool to room temperature, set aside.

[0076] The second, the configuration of multi-level structure nano golden flower growth liquid: get 0.4 milliliter, 24 mmol / liter of chloroauric acid aqueous solution and add in 35% polydiallyl dimethyl ammonium chloride (PDDA) aqueous solution, room temperature Stir for 15 minutes. Then add 0.8 ml, 0.1 mol / L aqueous ascorbic acid solution and stir for 30 seconds until the solution becomes colorless and transparent.

[0077] Third, nano-crystal seeds grow i...

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Abstract

The invention discloses a multistage nano golden flower, a preparation method and an application thereof. According to one embodiment, the preparation method comprises the steps of fully mixing chloroauric acid and poly diallyldimethylammonium chloride in an aqueous system, continuously stirring at room temperature until a prepared mixed solution is yellow, then adding ascorbic acid to the obtained yellow mixed solution, violently stirring, then adding a nano golden ball, quickly stirring, and then standing at room temperature. The preparation method has the advantages of simplicity, easiness in operation, good controllability and high productivity, a nano tip bulge is formed on the surface of the obtained multistage nano golden flower, and the multistage nano golden flower has a large specific surface area. The nano golden flower can be used as a gold-labelled antibody of an immunochromatographic strip, so as to be applied to the field of food safety, environment, medical diagnosis and the like, simple and high-sensitive analysis and detection can be performed on target objects, for example, high-sensitive detection of colibacillus can be performed, and the lower limit of the detection concentration reaches 103 CFU / mL.

Description

technical field [0001] The invention relates to a nano-golden flower with a multi-level structure, its preparation method and application, such as the application in the preparation of a high-sensitivity detection kit based on an immune test strip, and belongs to the technical field of material science and analysis and detection. Background technique [0002] As an extremely important member of the nanoparticle system, gold nanoparticles also have unique physical and chemical properties, such as good biocompatibility, making gold nanoparticles widely used in biomarkers and biosensing. At present, the preparation methods of nano gold mainly include: liquid phase reduction method, photochemical method, gas phase evaporation method, seed crystal growth method, electrochemical reduction method, phase transfer method, hydrothermal method, microwave method, etc. However, most of these methods can only be used to synthesize spherical gold nanoparticles, and are not suitable for pre...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00G01N33/569G01N33/558B82Y30/00B82Y40/00
Inventor 黄又举陈涛张磊
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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