A kind of separation detection method of pyrimethamine

A pyrimethamine and detection method technology, applied in the field of organic chemistry and analytical chemistry, can solve the problems of cumbersome operation, high column pressure, limiting the promotion and application of molecular imprinting-solid phase extraction, etc., and achieves mild reaction conditions, rapid reaction, Effect of improving adsorption capacity and selectivity

Active Publication Date: 2018-08-28
FOSHAN UNIVERSITY
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  • Claims
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Problems solved by technology

The production of solid phase extraction columns in this way is not only cumbersome to operate, but also has high column pressure and low flow rate during the extraction process. These disadvantages largely limit the further promotion and application of molecular imprinting-solid phase extraction.

Method used

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  • A kind of separation detection method of pyrimethamine
  • A kind of separation detection method of pyrimethamine

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Embodiment Construction

[0027] A method for separation and detection of pyrimethamine disclosed by the invention comprises the following steps:

[0028] (1) Sample treatment: extract the pyrimethamine in the sample and use it as the sample solution to be tested.

[0029] (2) Add pyrimethamine molecularly imprinted polymer to the test solution, shake and centrifuge to separate the pyrimethamine molecularly imprinted polymer.

[0030] (3) Elute the pyrimethamine in the pyrimethamine molecularly imprinted polymer with a certain volume of elution solvent, and collect the eluate.

[0031] (4) The concentration of pyrimethamine in the eluate was determined by HPLC / UV.

[0032] In this embodiment, a fish sample is taken as an example. Take 2g of fish sample, shred it and homogenize it, add 4g of anhydrous sodium sulfate and 10mL of acetonitrile-chloroform mixture with a volume ratio of 5:5 to the homogenized sample, mix well, centrifuge at 5000r / min for 5min, and take For the organic phase, the residue w...

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Abstract

The invention discloses a separation detection method for pyrimethamine. The separation detection method for pyrimethamine comprises the following steps that firstly, a sample is treated, and a solution to be detected is obtained; secondly, a pyrimethamine molecularly-imprinted polymer is added into the solution to be detected and is separated through centrifuging; thirdly, pyrimethamine is eluted with a certain volume of an eluting solvent, and eluant is collected; fourthly, the concentration of pyrimethamine in the eluant is measured with HPLC / UV, pyrimethamine serves as a template of the pyrimethamine molecularly-imprinted polymer, functional monomers silicon dioxide microspheres with grafted double bonds, a sulphydryl cross-linking agent and the like are added, and pyrimethamine is obtained through a sulphydryl-alkenyl click reaction. According to the pyrimethamine molecularly-imprinted polymer, the sulphydryl cross-linking agent is used in the preparing process, the sulphydryl-alkenyl click chemistry is used in combination with the molecular imprinting technology, the reaction conditions are mild, and the adsorption capacity and the selective performance to pyrimethamine are improved by optimizing the reaction conditions of synthesizing the molecularly-imprinted polymer through the sulphydryl-alkenyl click reaction.

Description

technical field [0001] The invention relates to the fields of organic chemistry and analytical chemistry, in particular to a separation and detection method for pyrimethamine. Background technique [0002] Pyrimethamine is also widely used in animal products and aquatic product breeding. Appropriate use can enhance the disease resistance of animals. It is a broad-spectrum antibacterial veterinary drug, mainly used to prevent chicken coccidiosis, fowl cholera and pullorum of piglets, etc. . However, pyrimethamine has a high degree of accumulation in aquatic products. If it exceeds a certain range, human consumption will destroy the human hematopoietic system, cause hemolytic anemia, and even cause potential carcinogenicity. At the same time, it has a direct effect on the central nervous system. Toxic effects. The United Kingdom and the United States have already clearly stipulated that pyrimethamine and other feed additives are prohibited as feed additives. Japan has implem...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G75/045C08J9/26B01J20/26B01J20/30G01N30/02C07D239/48
CPCB01J20/268C07D239/48C08G75/045C08J9/26C08J2201/0424C08J2381/02G01N30/02G01N2030/027
Inventor 陈忻陈晓刚梁勇詹根权
Owner FOSHAN UNIVERSITY
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