Mangosteen rind and gingerol antioxidant additive and preparation method thereof
An anti-oxidant additive, gingerol technology, is applied in the direction of food ingredients as antioxidants, the function of food ingredients, and food ingredients containing natural extracts. Toxic and side effects, good antioxidant effect, effect of reducing oxidative stress
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Embodiment 1
[0032] (1) Preparation of mangosteen bark extract:
[0033] a. After drying and pulverizing 1 kg of mangosteen bark, add 4 kg of 10% ethanol aqueous solution, heat reflux extraction at 50° C. for 40 minutes, and filter and concentrate to obtain the extraction solution;
[0034] b. Extracting the above extract with petroleum ether to obtain an extract;
[0035] c. The above extract is subjected to column chromatography with a polyethylene glycol 20000 column, and then eluted with a 5% aqueous ethanol solution to obtain a first-stage eluent;
[0036] d, the above-mentioned first-stage eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 1% acetic acid aqueous solution to obtain a second-stage eluent;
[0037] e, recrystallizing the above-mentioned second-stage eluent with ethanol, and drying to obtain the mangosteen bark extract;
[0038] (2) Mix 20 grams of mangosteen bark extract, 3 grams of gingerol, 0.01 grams of sodium chloride, 0...
Embodiment 2
[0040] (1) Preparation of mangosteen bark extract:
[0041] a. After drying and pulverizing 1 kg of mangosteen bark, add 4.5 kg of 15% ethanol aqueous solution, heat reflux extraction at 55° C. for 50 minutes, and filter and concentrate to obtain the extract;
[0042] b. Extracting the above extract with petroleum ether to obtain an extract;
[0043] c. Carry out column chromatography on the above-mentioned extract with a polyethylene glycol 20000 column, and then elute with an 8% aqueous ethanol solution to obtain the first-stage eluent;
[0044] d. The above-mentioned first-stage eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 2% acetic acid aqueous solution to obtain a second-stage eluent;
[0045] e, recrystallizing the above-mentioned second-stage eluent with ethanol, and drying to obtain the mangosteen bark extract;
[0046] (2) Mix 25 grams of mangosteen bark extract, 4 grams of gingerol, 0.015 grams of sodium chloride, 0...
Embodiment 3
[0048] (1) Preparation of mangosteen bark extract:
[0049] a. After drying and pulverizing 1 kg of mangosteen bark, add 5 kg of 20% ethanol aqueous solution, heat reflux extraction at 60° C. for 60 minutes, and filter and concentrate to obtain the extraction solution;
[0050] b. Extracting the above extract with petroleum ether to obtain an extract;
[0051] c. Carry out column chromatography on the above-mentioned extract with a polyethylene glycol 20000 column, and then elute with a 10% aqueous ethanol solution to obtain the first-stage eluent;
[0052] d, the above-mentioned first-level eluent is chromatographed with 300 mesh polyamide powder as an adsorbent, and then eluted with 3% acetic acid aqueous solution to obtain a second-level eluent;
[0053] e, recrystallizing the above-mentioned second-stage eluent with ethanol, and drying to obtain the mangosteen bark extract;
[0054] (2) Mix 30 grams of mangosteen bark extract, 5 grams of gingerol, 0.02 grams of sodium ch...
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