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Quantum dot and immunochromatography test strip rapid detection method for beta-stimulant multiresidue

A technology of immunochromatography and detection method, which is applied in the field of rapid detection of quantum dot immunochromatographic test strips with multi-residue β-agonists, can solve the problem of quantitative analysis of samples that are difficult to detect, and the inability to screen multiple β-agonists. and other problems, to achieve the effect of wide excitation wavelength range, convenient operation and accurate results

Active Publication Date: 2016-11-09
广州万联生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the immunochromatographic test strips reported so far can only detect one or two β-agonist drugs, and cannot screen multiple β-agonists at the same time, and can only achieve qualitative detection, which is difficult to detect. Samples for Quantitative Analysis

Method used

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  • Quantum dot and immunochromatography test strip rapid detection method for beta-stimulant multiresidue
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  • Quantum dot and immunochromatography test strip rapid detection method for beta-stimulant multiresidue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1 Quantum dot immunochromatographic test strip rapid detection method with multiple residues of β-agonists

[0073] A method for rapid detection of quantum dot immunochromatographic test strips with multiple residues of β-agonists, comprising the following steps:

[0074] (1) Preparation of quantum dot fluorescent probe: Dissolve 20 μL of quantum dots in 3.2 mL of 0.01mol / L pH7.4 phosphate buffer, add coupling agent 1-(3-dimethylpropyl)-3- Ethylcarbodiimide hydrochloride, N-hydroxysuccinimide solution, stirred at room temperature for 15 minutes, then added 50 μg β-agonist monoclonal antibody, stirred at room temperature for 1.5 hours, continued to add 0.4 mL of 10% BSA solution to block After reacting for 1 hour, centrifuge the reaction solution at 12,000 r / min for 40 minutes, remove the supernatant, redissolve the precipitate with reconstitution solution, and obtain the labeled complex of quantum dots and β-agonist monoclonal antibody, and store in the dark at ...

Embodiment 2

[0101] Example 2 Taking the standard clenbuterol hydrochloride (CL) as an example to establish a standard curve for quantum dot immunochromatography test strips

[0102] 1. Experimental materials

[0103] (1) β-agonist broad-spectrum monoclonal antibody, the preparation method is the same as in Example 1.

[0104](2) Goat anti-mouse IgG secondary antibody is a commercial reagent, purchased from Guangzhou Youkangduo Biotechnology Co., Ltd.

[0105] The preparation of the immunochromatographic test strip is the same as in Example 1.

[0106] 2. Configuration of CL standard solution, using Tris-HCl to prepare a series of standard solutions with concentrations of 0, 0.0156, 0.0625, 0.250, 1.00, 4.00, 16.00 and 64.0 μg / L, respectively, for test strip detection.

[0107] 3. Determination of test strips

[0108] Each concentration was tested 3 times, and after 10 min, the test strip FI was read with a fluorescent immunochromatography reader. T / FI C ratio. to FI T / FI C is th...

Embodiment 3

[0110] The precision of embodiment 3 quantum dot immunochromatography test strips

[0111] 1. Taking CL as an example, use different batches of test strips to test standard solutions with different CL contents, repeat the detection 3 times for each concentration, measure 3 different batches, and calculate the actual detection value.

[0112] 2. As shown in Table 1, the inter-assay and intra-assay coefficients of variation of test strip test results are both less than 15%, indicating that the method has good repeatability.

[0113] Table 1 Intra-assay and inter-assay variation of quantum dot immunochromatographic test strip detection methods

[0114]

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Abstract

The invention discloses a quantum dot and immunochromatography test strip rapid detection method for beta-stimulant multiresidue. The detection method includes the steps that a quantum dot is coupled with beta-stimulant monoclonal antibody to obtain a quantum dot and beta-stimulant monoclonal antibody labeling complex which is namely a quantum dot fluorescence probe; after the labeling complex serving as the quantum dot fluorescence probe and a sample to be detected are mixed uniformly and incubated, and an immunochromatography test strip is used for detecting the beta-stimulant residue. Multiple beta-stimulant drugs can be screened fast, the detection method is simple, fast, accurate in result, high in sensitivity, low in prices and wide in application range and has excellent application and popularization prospects, and qualitative and quantitative determination can be carried out.

Description

technical field [0001] The invention belongs to the technical field of food safety immune detection. More specifically, it relates to a rapid detection method for a quantum dot immunochromatographic test strip with multiple residues of β-agonists. Background technique [0002] β-agonists are a class of phenethylamines that are similar in chemical structure and physiological function to epinephrine and norepinephrine. β-agonists are commonly used clinically to prevent and treat diseases such as bronchospasm and asthma in humans and animals. Large doses of β-agonists can affect the redistribution of nutrients in animals, accelerate fat catabolism, increase protein synthesis, and significantly increase lean meat percentage. Therefore, β-agonists have been widely used as feed additives in livestock production. However, β-stimulants accumulate seriously in the viscera of animals and can enter the human body through the food chain, causing symptoms such as palpitations, dizzine...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N33/577G01N33/558G01N33/533
CPCG01N33/02G01N33/533G01N33/558G01N33/577
Inventor 杨金易曾道平朱彬
Owner 广州万联生物科技有限公司
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