Establishing method of lung cancer animal model with inflammation foundation

Abstract

The invention provides an establishing method of a lung cancer animal model with an inflammation foundation. The method includes the following steps of firstly, establishing pBabe-CMV-EGFP-puro plasmids; secondly, establishing an EGFP over-expression system mediated by reverse transcription viruses; thirdly, establishing lung cancer cell lines for stably expressing EGFP; fourthly, establishing an inflammation model; fifthly, establishing the lung cancer animal model with the inflammation foundation, wherein 1*107 lung cancer cell lines stably expressing the EGFP are prepared into a cell suspension, 75 microliters of cell suspension is injected into a left lung of a naked mouse with lung inflammation from the gap of a back rib through an insulin syringe, the mouse is fed continuously for 2 weeks, and then the lung cancer animal model with the inflammation foundation is obtained. The EGFP used in the establishing method can produce fluorescent light, light-emitting strength can be detected by a corresponding instrument device, and therefore the existence of lung cancer can be proved.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for establishing an animal model of lung cancer with an inflammation basis. Background technique [0002] Epidemiological and clinical studies have confirmed that approximately 25% of adult tumors are caused by chronic inflammation. For example, chronic viral or bacterial infections of the stomach, liver, and cervix, as well as long-term exposure of the lungs to tobacco smoke or other environmental compounds, will significantly promote the chronic tissue damage of these organs and subsequent tumors. [0003] Although the relationship between inflammation and cancer has been clarified and it is called "the seventh characteristic of cancer", the underlying mechanism of chronic inflammation in cancer is still unclear. The molecular mechanism of chronic inflammation promoting tumor cell migration, invasion and metastasis It remains to be studied in depth. The current rese...

AI Technical Summary

Problems solved by technology

[0006] Technical problem to be solved: Aiming at the disadvantage that the existing porcine pancreatic elastase commonly used for inducing lung chronic inflammation can lead to the degradation of structural proteins in the lung, and then lead to emphysema and chronic obstructive pneumonia, the present invention provides an inflammatory-based elastase A method for establishing an animal model of lung cancer, which combines the nude mouse lung orthotopic transplantation model with the PPE-induced chronic pneumonia model, and successfully established a lung cancer model with an inflammatory basis

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