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Application of aconite polysaccharide in the preparation of drugs for treating atherosclerosis

A technology of atherosclerosis and aconite polysaccharide, which is applied in the application field of aconite polysaccharide in the preparation of drugs for the treatment of atherosclerosis, which can solve problems such as adverse reactions, patient death, liver function damage, etc., and achieve reduction of morbidity and good research Foreground, High Confidence Effects

Inactive Publication Date: 2020-04-14
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) Vasomodulatory drugs, including antiplatelet drugs and vasomotor drugs, these drugs have no therapeutic significance on lipids in AS plaques
(3) Antioxidant drugs, including probucol, etc. These drugs inhibit LDL oxidation and improve the condition of AS are still in the research stage
(4) Although there are many new anti-AS drugs being studied, there are not many ideal drugs, most of which are expensive and have some adverse reactions
Take statins, which are currently the most widely used and most effective, can cause liver transaminases to increase, and the possibility of liver function damage after long-term medication is greater. The most serious side effect is rhabdomyolysis, which can cause death in severe cases.

Method used

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  • Application of aconite polysaccharide in the preparation of drugs for treating atherosclerosis
  • Application of aconite polysaccharide in the preparation of drugs for treating atherosclerosis
  • Application of aconite polysaccharide in the preparation of drugs for treating atherosclerosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Extraction of aconite polysaccharide

[0033] Take 100 grams of dried aconite and crush it into fine particles, degrease with 95% ethanol, then extract with 1 liter of double distilled water at 90-100°C for 2 hours and filter. The residue was further extracted with 750ml of water for 1 hour. The combined aqueous extracts were concentrated with a rotary evaporator under reduced pressure (50°C) and filtered. The filtrate was mixed with 4 times the volume of 95% ethanol at 4°C, and centrifuged at 5000 rpm for 20 minutes. The precipitate was redissolved in 300ml of water, and deproteinized three times with 60ml of a mixture of chloroform and n-butanol at a volume ratio of 5:1. The deproteinized extract was dialyzed in double distilled water for three days, and then 3 times the volume of 95% ethanol was added for alcohol analysis. The precipitate was washed with absolute ethanol, and then vacuum-dried at 40° C. to obtain crude polysaccharide for future use.

Embodiment 2

[0034] Example 2 Establishment of Animal Model of Atherosclerosis

[0035] ApoE gene knockout mice were selected and fed with high-fat animal feed, which was made from ordinary mouse feed with fat and cholesterol (containing 21% fat and 0.15% cholesterol, w / w), and sterilized by radiation. Mice were housed in the school laboratory animal center.

[0036] The apoE gene knockout mice were randomly divided into control group, simvastatin prevention group, simvastatin prevention high (200 mg), medium (100 mg), low (50 mg) dose group and aconite polysaccharide prevention group, aconite polysaccharide treatment high ( 200mg), medium (100mg), low (50mg) dose groups and 9 groups of animals. In each group of aconite polysaccharide and simvastatin, the animals were administered by intragastric administration, and the control group was intragastrically administered with the same dose of drinking water, once a day, and the experiment ended after 12 weeks. The aconite polysaccharide prev...

Embodiment 3

[0039]Example 3 Prevention and treatment effect of aconite polysaccharide on atherosclerosis

[0040] Oil red 0 staining to detect the area of ​​atheromatous plaque:

[0041] Animals were anesthetized and decapitated, and the aorta was dissected under a microscope, fixed in 10% formaldehyde for 24 hours, stained with 0.3% Oil Red 0 staining solution, unstained Oil Red 0 was removed with 60% isopropanol, and counterstained with hematoxylin , 1% HCl color separation and return to blue.

[0042] Red atherosclerotic plaques at the aortic root and aortic arch were observed under a microscope, and the percentage of aortic atherosclerotic plaque area to the total aortic intima area was analyzed and counted by an automatic image analysis system.

[0043] The apoE gene knockout mice were selected for the pre-experiment, and they were kept in separate cages in a quiet and clean environment. The indoor temperature was controlled at 24±2, and clean drinking water and special feed were gi...

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Abstract

The present invention relates to the application of aconite polysaccharide in the preparation of drugs for treating atherosclerosis, the application is the application of aconite polysaccharide in the preparation of drugs for improving the activity of tissue antioxidant enzymes SOD, CAT, GSH-Px; the application can also be Application of aconite polysaccharide in preparation of medicine for reducing MDA content in tissues. The present invention firstly applies the aconite polysaccharide monomer in the preparation of medicines for treating atherosclerosis, and the prepared medicines can effectively reduce the incidence of atherosclerosis.

Description

technical field [0001] The invention belongs to the field of pharmacy and pharmacy, and in particular relates to the application of aconite polysaccharide in preparing medicine for treating atherosclerosis. Background technique [0002] Atherosclerosis (AS) is the main pathological basis of cardiovascular and cerebrovascular diseases such as coronary heart disease and stroke, and is a major disease that endangers human health. With the improvement of living standards and changes in lifestyle, cardiovascular and cerebrovascular diseases caused by AS have become one of the diseases that seriously endanger human health, and the incidence of atherosclerosis in my country is also increasing year by year. Age tends to be younger. The United States, Europe and Japan died of the disease accounted for about 50% of the total number of deaths. In recent years, the incidence of the disease has also increased significantly in our country. According to the results of autopsy, the detect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P9/10A61K36/714
CPCA61K31/715A61K2236/331A61K2236/333A61K2236/51A61K2236/53
Inventor 刘颖廖丽贞段新芬
Owner GUANGDONG PHARMA UNIV
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