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A kind of bacillus subtilis and its application in kitchen waste

A technology for Bacillus subtilis and kitchen waste, applied in the field of microorganisms, can solve problems such as poor effect and low efficiency, and achieve the effects of good treatment effect, good development prospect, and efficient, fast and stable treatment method.

Active Publication Date: 2019-12-13
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing biological treatment methods have low efficiency and poor effect

Method used

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  • A kind of bacillus subtilis and its application in kitchen waste
  • A kind of bacillus subtilis and its application in kitchen waste
  • A kind of bacillus subtilis and its application in kitchen waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and screening of Bacillus subtilis (Bacillus subtilis) CY-4

[0033] The separation process of Bacillus subtilis (Bacillus subtilis) CY-4 of the present invention: take Zhenjiang Jurong planting grape rhizosphere soil 10g, add 90mL sterile water, obtain 10 -1 Dilution, in a shaker at 30°C at 130r / min, cultured for 2-3h, continued to add sterile water to dilute to obtain 10 -5 , 10 -6 , 10 -7 , 10 -8 200uL of the above dilutions were spread on LB plates, cultured at 30°C for 48 hours, and a single colony was picked and inoculated on the slant of LB solid medium for preservation.

[0034] The physiological activity characteristics of Bacillus subtilis CY-4 are as follows:

[0035] a. Morphological characteristics: Gram staining is positive, rod-shaped, with spores, and the spores are oval. On the LB solid medium, the surface of the colony is rough and wrinkled, opaque, with irregular edges, milky white to slightly yellow;

[0036] b. Physiologica...

Embodiment 2

[0042] Example 2 Preparation of a biological treatment agent containing Bacillus subtilis and its application in kitchen waste

[0043] Preparation of biological treatment agent containing Bacillus subtilis:

[0044] (1) Cultivate Bacillus subtilis on LB solid culture at 30°C for 24h; inoculate it in a fermentation liquid shaker flask, and culture it with shaking at 30°C and 130r / min for 24h to form a seed liquid;

[0045] The seed solution is inoculated into the culture material in the fermenter at a volume ratio of 1:400 for fermentation production. The basic working parameters of the fermenter are: temperature: 30-35°C, stirring speed: 130r / min, pH=6.6-7.2 , dissolved oxygen: 30%, tank pressure: 0.04MPa; stop fermentation after 24 hours of fermentation, then centrifuge at 6000r / min for 10 minutes, take the sediment and dilute it with sterile water to make bacterial agent; the concentration of viable bacteria in the bacterial agent is 6.3 ×10 9 CFU / mL;

[0046] (2) Sawdus...

Embodiment 3

[0056] Example 3 Preparation of Biological Treatment Agent Containing Bacillus subtilis Inoculum and Application in Kitchen Waste

[0057] This embodiment is basically the same as Example 2, except that in step (2), the mass ratio of sawdust, bran powder and rice husk is 8:1:1; in step (3), microbial inoculum and carrier The mass ratio is 2:15.

[0058] Weight loss efficiency test of biological treatment agent on food waste after treatment:

[0059] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0060] (2) The biological treatment agent prepared by the above method is applied to the treatment of kitchen waste: preheating operation 6h, ventilation and stirring at the same time; 10kg of food waste to be treated is added in the processor after the pre-operation, and biological treatment agent Mix for aerobic fermentation, and ventilate and stir at the same time; the amount of biological treatment a...

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Abstract

The invention provides a kind of Bacillus subtilis, which is classified as Bacillus subtilis (Bacillus subtilis) CY‑4, preserved in the General Microbiology Center of China Microbial Strain Preservation Management Committee, and the address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, postcode 100101, postcode 100101, deposit number: CGMCC NO.12447, deposit date is May 13, 2016. The invention also provides the application of the bacillus subtilis in the treatment of kitchen waste. The Bacillus subtilis has high activity of protease, amylase and oil hydrolase, degrades glucose to produce acid, and is a thermophilic bacteria with high salt tolerance and acid resistance. Through effective combination with the carrier, it can be quickly and efficiently put into the kitchen The weight reduction reaches 85%.

Description

technical field [0001] The invention belongs to the technical field of microbes, in particular to a bacillus subtilis, and also relates to the application of the bacillus subtilis in the treatment of kitchen waste. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incineration, dehydration, va...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14B09B3/00C12R1/125
CPCB09B3/00C12N1/20C12N11/14C12N1/205C12R2001/125
Inventor 周哲刘立鹤王哲李浩张炳如杜国扬
Owner BIOMAX ECOLOGICAL ENG
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