Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel TRAIL fusion protein

A fusion protein and protein technology, which can be used in peptide/protein components, animal/human proteins, targeting specific cell fusion, etc., and can solve the problem of not having cell division and proliferation activity.

Active Publication Date: 2016-10-26
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The third loop region of TGF-α (TGF3L) can bind to EGFR, but it does not have the mitogenic and proliferative activity of TGF-α and EGF[27]

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel TRAIL fusion protein
  • Novel TRAIL fusion protein
  • Novel TRAIL fusion protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0052] The purpose of the present invention is to provide a new type of TRAIL fusion protein, a method for preparing a new type of TRAIL fusion protein, its coding gene, and expression vector, the steps of expressing the fusion protein coding gene and expression vector, and a new type How to use TRAIL fusion protein.

[0053] The present invention found that TRAIL protein is connected with the third loop region of TGF-α (TGF3L) to form a new fusion protein, which has a stronger tumor cell killing effect than TRAIL protein; this enhancement effect is mainly reflected in tumor cells sensitive to TRAIL , And normal cells that are not sensitive to TRAIL toxicity are still inactive. For many TRAIL-sensitive human tumor cells, the specific tumor-killing effect of the fusion protein can be increased by a hundred-fold or even a thousand-fold; for mouse tumor L929 cells, the tumor-killing effect of the fusion protein can still be increased by more than one hundred-fold. The extremely hig...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a novel TRAIL fusion protein, which belongs to the field of biotechnology. The novel TRAIL fusion protein contains a third loop sequence of TGF-alpha and has strong specific tumor killing effect. The invention also discloses a coding gene of the TRAIL fusion protein and an expression pathway thereof, a production method for the fusion protein and application of the fusion protein in treatment of diseases like tumors.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to a novel TRAIL fusion protein, a method for producing the fusion protein using genetic engineering technology, and its application as a medicine to treat tumors and other related diseases. Background technique [0002] TRAIL (TNF-related apoptosis-inducing ligand), also known as Apo2L (Apo-2ligand, apoptin-2 ligand), is a member of the tumor necrosis factor superfamily [1] , It was officially named Tumor necrosis factor ligand superfamily member 10 (Tumor necrosis factor ligand superfamily member 10). TRAIL molecule is composed of 281 amino acid residues, is a typical type II transmembrane protein, 18-38 amino acid residues form a single transmembrane region with a helical structure, the amino terminal is located in the cytoplasm, and the carboxyl segment is 39-281 Amino acid residues are located outside the cell. The extracellular part of TRAIL can be cleaved by proteases and released int...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K19/00C12N15/62A61K38/17A61K47/48A61P35/00
CPCA61K38/00C07K14/495C07K14/70575C07K2319/33
Inventor 王楠闫征王燕
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com