A kind of method for indoor seedling cultivation of naked starworm
A technology of naked star worms and seedlings, applied in the field of aquaculture, can solve the problems of low yield and short cultivation time, and achieve the effects of improving survival rate, easy operation, and increasing seedling output
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Embodiment 1
[0026] On May 11, 2014, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:
[0027] (1) Parent selection: Healthy individuals were selected as parents from the cultured colony of P. phalaensis in Donghai Island, Zhanjiang City, with a size of (16±4.3) g / tail, and a female:male ratio of 10:1.
[0028] (2) Induce parental release of gametes: first, soak the selected parents in 0.05% 5-HT solution for 10 minutes, and dry them in the shade for 40 minutes; put them into the parent pool to induce production, the thickness of the sand layer in the parent pool is 10 cm, and the diameter of the sand grains is 200-2000 µm , the water temperature is (28±2.3) ℃, and the fertilization rate is 86%.
[0029] (3) Observing at any time after fertilization, it was found that the fertilized eggs began to hatch into sea ball larvae at 33 hours. At this time, they were fed with Isoflagellates Zhanjiang, Cloud Microalgae and Chlorella, and the...
Embodiment 2
[0035] On May 2, 2015, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:
[0036] (1) Selection of parents: Healthy starworms were selected from the breeding population in Caotan Town, Suixi County, Zhanjiang City as parents, with a size of (18±3.2) g / tail, and a female:male ratio of 7:1.
[0037] (2) Induce the parents to release gametes: Soak the selected parents in 0.07% 5-HT solution for 15 minutes, dry them in the shade for 30 minutes, and put them into the parent pond to induce production. The water temperature is (29±1.2) ℃, and the fertilization rate is 90%.
[0038] (3) Observe at any time after fertilization. After 40 hours, the primary sea ball larvae hatched, and the hatching rate was 92%. 4 pieces / ml.
[0039] (4) Early larvae cultivation: transfer the siphon seaball larvae to the larvae pool, the density of the larvae is 0.6 / ml, and feed them with Zhanjiang Isoflagellates, cloud microalgae and chlorella, ...
Embodiment 3
[0044] On June 10, 2015, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:
[0045] (1) Selection of parents: Select healthy P. glabrata as parents from the breeding population in Caotan Town, Suixi County, Zhanjiang City, with a size of (17±4.2) g / tail, and a female:male ratio of 8:1.
[0046] (2) Induce the parents to discharge gametes: Soak the parents in 0.10% 5-HT solution for 20 minutes, and dry them in the shade for 50 minutes; then put them into the parent pool to induce production. (29±2.2) ℃, the fertilization rate is 80%.
[0047] (3) Observe at any time after fertilization. After 30 hours, the primary sea ball larvae hatched, with an average body length of 260um. At this time, they were fed with Zhanjiang Isoflagellates, cloud microalgae and chlorella, and the daily feeding amount was 1.5×10 4 pieces / ml.
[0048] (4) Early larvae cultivation: absorb larvae and transfer them to the larvae pool; the density o...
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