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Method for cultivating larvae of sipunculus nudus indoors

A technology for naked star worms and seedlings is applied in the field of aquaculture, which can solve the problems of low yield and short cultivation time, and achieve the effects of improving the survival rate, easy operation and improving seedling output.

Active Publication Date: 2016-10-26
湛江市碧海湾水产科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cultivation of starworm seedlings mainly includes outdoor earthen pond cultivation and indoor cement pond cultivation, of which the indoor cement pond cultivation technology mainly involves parental cultivation, larvae cultivation and seedling cultivation; compared with outdoor earthen pond cultivation, indoor cement pond cultivation is mainly through control In terms of parental maturation promotion, larvae density, bait and breeding management, etc., the growth rate, survival rate and metamorphosis rate of larvae are improved, thereby improving seed production and economic benefits. However, there are defects of low yield and short cultivation time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] On May 11, 2014, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:

[0027] (1) Parent selection: Healthy individuals were selected as parents from the cultured colony of P. phalaensis in Donghai Island, Zhanjiang City, with a size of (16±4.3) g / tail, and a female:male ratio of 10:1.

[0028] (2) Induce parental release of gametes: first, soak the selected parents in 0.05% 5-HT solution for 10 minutes, and dry them in the shade for 40 minutes; put them into the parent pool to induce production, the thickness of the sand layer in the parent pool is 10 cm, and the diameter of the sand grains is 200-2000 µm , the water temperature is (28±2.3) ℃, and the fertilization rate is 86%.

[0029] (3) Observing at any time after fertilization, it was found that the fertilized eggs began to hatch into sea ball larvae at 33 hours. At this time, they were fed with Isoflagellates Zhanjiang, Cloud Microalgae and Chlorella, and the...

Embodiment 2

[0035] On May 2, 2015, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:

[0036] (1) Selection of parents: Healthy starworms were selected from the breeding population in Caotan Town, Suixi County, Zhanjiang City as parents, with a size of (18±3.2) g / tail, and a female:male ratio of 7:1.

[0037] (2) Induce the parents to release gametes: Soak the selected parents in 0.07% 5-HT solution for 15 minutes, dry them in the shade for 30 minutes, and put them into the parent pond to induce production. The water temperature is (29±1.2) ℃, and the fertilization rate is 90%.

[0038] (3) Observe at any time after fertilization. After 40 hours, the primary sea ball larvae hatched, and the hatching rate was 92%. 4 pieces / ml.

[0039] (4) Early larvae cultivation: transfer the siphon seaball larvae to the larvae pool, the density of the larvae is 0.6 / ml, and feed them with Zhanjiang Isoflagellates, cloud microalgae and chlorella, ...

Embodiment 3

[0044] On June 10, 2015, the indoor seedling cultivation of the naked star worm was carried out. The specific steps are as follows:

[0045] (1) Selection of parents: Select healthy P. glabrata as parents from the breeding population in Caotan Town, Suixi County, Zhanjiang City, with a size of (17±4.2) g / tail, and a female:male ratio of 8:1.

[0046] (2) Induce the parents to discharge gametes: Soak the parents in 0.10% 5-HT solution for 20 minutes, and dry them in the shade for 50 minutes; then put them into the parent pool to induce production. (29±2.2) ℃, the fertilization rate is 80%.

[0047] (3) Observe at any time after fertilization. After 30 hours, the primary sea ball larvae hatched, with an average body length of 260um. At this time, they were fed with Zhanjiang Isoflagellates, cloud microalgae and chlorella, and the daily feeding amount was 1.5×10 4 pieces / ml.

[0048] (4) Early larvae cultivation: absorb larvae and transfer them to the larvae pool; the density o...

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Abstract

The invention discloses a method for cultivating larvae of sipunculus nudus indoors. The method mainly comprises the steps of parent selection, spawn induction, larval cultivation, nyad cultivation and the like. Compared with the prior art, the method has the beneficial effects that (1) the emission ratio and synchronicity of parent sperms and eggs can be improved by using a 5-hydroxytryptamine solution immersion and drying-in-the-shade stimulation combined induction method; (2) algae are fed when individual pelagospheric larvae start to hatch, so that the survival rate of pelagospheric larvae can be increased; (3) water is not changed in the process of cultivating larvae, and the water quality is controlled by reasonably controlling the larval density and the bait amount; (4) benthic diatom is cultivated in a larval pool in advance, sufficient nutrition is provided to larvae which are just attached, and the metamorphosis survival rate and growth rate of larvae can be increased; and (5) larval baits are subjected to superfine grinding, so that digestion of larvae can be benefited. The method can be used for improving the yield of the larvae so as to increase the economic benefit, and has the advantages of simple operation, easy popularization and the like.

Description

technical field [0001] The invention relates to aquaculture technology, more specifically, to a method for cultivating indoor seedlings of the naked star worm. Background technique [0002] naked starworm ( Sipunculus nudus ) is a common species in the phylum Astrozoa, living in caves in the intertidal zone, and is an important regional marine treasure. Affected by the rising market price, the fishing intensity of the naked star worm is increasing day by day, coupled with the change of the marine environment and the destruction of the tidal flats, the resources of the naked star worm have decreased significantly. At present, Beihai, Zhanjiang and other places have carried out asterworm breeding to varying degrees, but the source of seedlings still mainly depends on wild populations caught. The yield and quality of seedlings are very unstable and cause serious damage to natural resources. According to statistics, the suitable aquaculture area of ​​the naked starworm in Guan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K10/30A23K10/22
Inventor 王庆恒邓岳文张家炜杨创业杜晓东
Owner 湛江市碧海湾水产科技有限公司
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