Immunochromatography detection system and detection method

Abstract

The invention provides an immunochromatography detection system. The system comprises a light source for detection, a standard light source, a photoelectric detector, and a piece of test paper for detection; the test paper for detection is arranged in a detection area, a detection line is arranged in the detection area, when a detection target exists in a sample, an immunochromatography reaction is carried out for nano-gold marker particles, and the particles are aggregated on the detection line; the light source for detection and the standard light source can respectively emit detection light and reflected light to the test paper for detection; the photoelectric detector can receive the reflected light in the detection area; and the test paper for immunochromatography detection is provided with a calibration area, the calibration area is provided with a calibration color block, the calibration color block is positioned in a color developing range of the nano-gold marker particles, a detection result is corrected by means of irradiation on the calibration color block by the standard light and the light for detection, and whether further correction is needed is determined.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography detection system. Background technique [0002] Colloidal gold solution refers to the gold sol whose dispersed phase particle diameter is between 1-150nm, belongs to heterogeneous heterogeneous system, and the color is orange-red to purple-red. Colloidal gold can be used as a probe for precise positioning of cell surface and intracellular polysaccharides, proteins, peptides, antigens, hormones, nucleic acids and other biological macromolecules, and can also be used for daily immunodiagnosis. Because colloidal gold has many advantages as a marker, it has developed rapidly in many research fields at home and abroad since the technology came out. It has been widely used in the fields of electron microscopy, flow cytometry, western blotting, protein staining, and the manufacture of in vitro diagnostic reagents. The current application ...

AI Technical Summary

Problems solved by technology

However, the conventional technical solution does not consider the influence of aging of system components. In order to overcome the above-mentioned defects of the prior art, the invention patent of 201210185414.7 proposes a performance calibration method of the gold standard immunochromatographic test strip detection system. According to the data information of the gold standard color card when the system leaves the factory, the best response curve corresponding to the concentration of the detected substance is established according to this data information; in a specific time period, the data information corresponding to the color card is re-tested, and it is compared with the calibration Standards are compared to calculate a correction factor by which the result of aging of system components is corrected

However, the technical solution of this invention still has the following defects: on the one hand, it needs to be corrected at a specific interval, and it is impossible to judge whether it needs to be corrected in real time; on the other hand, its correction is only a numerical correction, without considering the specific physical process , the result is nonlinear and the accuracy is insufficient

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