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Rapid propagation method of Agrimonia pilosa

A Agrimony, fast technology, applied in the field of botany, can solve the problems of undisclosed operating conditions, etc., achieve the effect of simple and easy experimental operation, prevent vitrification, and increase the multiplication coefficient

Active Publication Date: 2016-09-28
SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned prior art mainly discloses the production of medicinal plant secondary metabolites (flavonoids) using tissue culture technology, and has not yet disclosed the specific operating conditions for tissue culture from the perspective of seedling production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In April, cut out no pests and diseases, strong branches of the year as explants, remove the leaves of the plants, apply detergent on the surface of the stems of the plants, scrub gently with gauze, and then wash them repeatedly with tap water until there is basically no foam, repeat The above cleaning steps were performed 3 times, and finally rinsed with running water for 3 hours. On the ultra-clean workbench, first cut the branches into about 2 cm stems with axillary buds, then soak them in 75% alcohol for 30 seconds, rinse them once with sterile water, then disinfect them with 0.1% mercury liter for 8 minutes, and rinse them with sterile water 6 times. The above-mentioned sterilized explants were inoculated into the culture medium, one in each bottle, and began to grow after 5 days of culture. The inoculation medium was MS+6-BA1.5 mg / L+NAA0.1 mg / L. When the stem segment axillary buds on the primary culture germinate and grow to 2-3cm, cut the axillary buds from the e...

Embodiment 2

[0025]In May, cut out no pests and diseases, strong branches of the year as explants; remove the leaves of the plants, apply detergent on the surface of the stems of the plants, gently scrub with gauze, and then repeatedly wash with tap water until there is basically no foam, repeat The above cleaning steps are performed 3 times; finally, rinse with running water for 5 hours. On the ultra-clean workbench, first cut the branches into stems with axillary buds of about 2 cm, then soak them in 75% alcohol for 30 seconds, rinse them once with sterile water, then disinfect them with 0.1% mercury liter for 12 minutes, and rinse them with sterile water six times. The above-mentioned sterilized explants were inoculated into the medium, one in each bottle, and started to grow after 7 days of culture. The inoculation medium was MS+6-BA2.0mg / L+NAA0.1mg / L. When the stem segment axillary buds on the primary culture germinate and grow to 2-3cm, cut the axillary buds from the explants and tra...

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Abstract

The invention relates to a rapid propagation method of Agrimonia pilosa. The rapid propagation method comprises the steps of shearing a thick and strong annual twig without diseases and insect pests as an explant, disinfecting the explant, inoculating the disinfected explant to a culture medium, culturing for 5-7 days, shearing auxiliary buds from the explant after auxiliary buds on a first-generation culture medium germinate and grow to be 2cm-3cm, transplanting the auxiliary buds to a first subculture medium, carrying out multiplication, after carrying out subculturing for 3-5 times, transferring the auxiliary buds into a second subculture medium for culture, transferring healthy and strong plantlets with 2-5 leaves with the leaf length of 3cm-6cm into a rooting culture medium, carrying out acclimatization and transplantation after the roots grow to be 1cm-3cm to germinate, transferring a culture bottle into a greenhouse, carrying out acclimatization for 4-10 days under natural conditions, taking out the acclimatized plantlets from the culture bottle, cleaning the culture medium adherent to the plantlets, soaking the roots into a Celest solution, taking out the roots, and transplanting the roots into a cultivation medium. By virtue of the rapid propagation method, the survival rate of transplanting of the Agrimonia pilosa is increased to be 92%-95%.

Description

technical field [0001] The invention belongs to the field of botany, and in particular relates to a plant tissue culture technique, in particular to a Agrimony, specifically a rapid propagation method of Agrimony. Background technique [0002] Agrimony ( Agrimonia pilosa ) is a perennial herbaceous plant belonging to the family Rosaceae, with a height of 50-120 cm. It is mainly produced in Zhejiang, Jiangsu, Hubei, Heilongjiang and other places. Because Agrimony has strong biological activity, scholars at home and abroad have studied its chemical composition and pharmacological effects for many years. Studies have shown that Agrimony contains flavonoids, tannins, glycosides, organic acids, volatile oils, triterpenoid saponins and other chemical components, which have various pharmacological effects such as anti-tumor, hypoglycemic, analgesic, anti-inflammatory, hemostatic, and anti-arrhythmic. With the continuous deepening of pharmacological and clinical research on Ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 罗玉兰朱春玲张冬梅沈烈英
Owner SHANGHAI ACADEMY OF LANDSCAPE ARCHITECTURE SCI & PLANNING
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