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A rapid detection method for volatile components in tobacco leaves

A technology of volatile components and detection methods, applied in the field of rapid detection of volatile components in tobacco leaves, can solve problems affecting accurate analysis, inconsistent retention time of FID signals, etc., achieve short time consumption, high efficiency of sample determination and analysis, and pretreatment simple effect

Active Publication Date: 2017-07-07
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

[0005] At present, two instruments are usually used for the above analysis, that is, a GC-QTOF MS instrument and a gas chromatograph equipped with an FID, or a chromatograph equipped with an FID coupled with a QTOF MS instrument. Among them, the chromatograph is equipped with two injection ports and connected to two columns. The above analysis methods all have the problem of inconsistency in the retention time of the mass spectrum signal and the FID signal of the same substance, which affects the accurate analysis.

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  • A rapid detection method for volatile components in tobacco leaves
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Embodiment 1

[0029] A rapid detection method for the volatile components of tobacco leaves, using dichloromethane to extract tobacco leaf powder samples, the extracted samples are separated by a chromatographic column, and the microplate flow control technology is used to control the end split of the chromatographic column, and at the same time enter the quadrupole flight Time-to-time mass spectrometer (QTOF MS) and flame ionization detector (FID) are used to measure the volatile components of tobacco leaves to achieve accurate qualitative and quantitative analysis; the specific steps are as follows:

[0030] (1) Sample pretreatment: Take 1 g of smoke powder, add 5 mL of dichloromethane solvent, and ultrasonicate for 10 min, then take the extract for analysis;

[0031] (2) Analysis of samples:

[0032] The microplate flow control technology is used to regulate the end split of the chromatographic column. According to the split ratio of 1:1, half of the samples enter the QTOF MS, and the ot...

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Abstract

A rapid detection method of tobacco leaf volatile components is characterized in that the method adopts dichloromethane for extraction of tobacco leaves, a sample after extraction is separated by a chromatographic column, a tail end shunt of the chromatographic column is regulated by using a micro plate flow path control technology, at the same time, the shunt enters a quadrupole time-of-flight mass spectrometer (QTOF MS) and a flame ionization detector (FID) and is determined, accurate qualitation and quantitation of the tobacco leaf volatile components is achieved, the whole process has the advantages of simple operation and short use time, and mass and automatic processing of the sample is achieved. The process can be completed by only requiring a simple solvent for extraction, a small amount of an organic solvent is consumed, the whole process has no second-order reaction problem of the tobacco leaf volatile components, and actual conditions of the sample is really reflected; an extract liquid is detected by using gas chromatography-quadrupole time-of-flight mass spectrometry / flame ionization detection for a first time, only one-needle sample injection is needed, and a QTOF MS spectrogram and an FID spectrogram are obtained, wherein QTOF MS can perform accurate quantitation of components of complex samples while the FID can perform accurate quantification of samples with wide concentration scope, and the retaining time of the QTOF MS spectrogram and the retaining time of the FID spectrogram are just the same.

Description

technical field [0001] The invention belongs to the technical field of measuring volatile components of tobacco leaves, and in particular relates to a rapid detection method for volatile components of tobacco leaves. Background technique [0002] The volatile components of tobacco leaves are important components of tobacco leaves, play a major role in the aroma of tobacco, and are an important factor in evaluating the grade of tobacco leaves. At present, there are many methods to report the analysis of the volatile components of tobacco leaves, usually using extraction techniques such as steam distillation, simultaneous distillation extraction and accelerated solvent extraction, the volatile components in the extracted tobacco leaves are analyzed by gas chromatography-mass spectrometry (GC-MS) Determination. The current pretreatment methods all have high extraction temperature, prone to secondary reaction problems, cannot truly reflect the actual volatile components in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/78
CPCG01N30/78G01N2030/027
Inventor 秦亚琼王晓瑜潘立宁崔华鹏贾云祯丁丽蔡君兰刘绍锋余晶晶张晓兵赵晓东王冰
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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