A rapid detection method for volatile components in tobacco leaves
A technology of volatile components and detection methods, applied in the field of rapid detection of volatile components in tobacco leaves, can solve problems affecting accurate analysis, inconsistent retention time of FID signals, etc., achieve short time consumption, high efficiency of sample determination and analysis, and pretreatment simple effect
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[0029] A rapid detection method for the volatile components of tobacco leaves, using dichloromethane to extract tobacco leaf powder samples, the extracted samples are separated by a chromatographic column, and the microplate flow control technology is used to control the end split of the chromatographic column, and at the same time enter the quadrupole flight Time-to-time mass spectrometer (QTOF MS) and flame ionization detector (FID) are used to measure the volatile components of tobacco leaves to achieve accurate qualitative and quantitative analysis; the specific steps are as follows:
[0030] (1) Sample pretreatment: Take 1 g of smoke powder, add 5 mL of dichloromethane solvent, and ultrasonicate for 10 min, then take the extract for analysis;
[0031] (2) Analysis of samples:
[0032] The microplate flow control technology is used to regulate the end split of the chromatographic column. According to the split ratio of 1:1, half of the samples enter the QTOF MS, and the ot...
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