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Bacillus subtilis SE201412 and organic-selenium-rich bactericide

A technology of SE201412 and Bacillus subtilis, which is applied in the field of microorganisms, can solve the problems of not producing water-soluble organic selenium, and cannot directly produce water-soluble organic selenium, and achieve the effect of improving quality and increasing yield

Active Publication Date: 2016-09-21
HUBEI UNIV FOR NATITIES +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the selenium-enriched yeast currently produced in the market cannot directly produce water-soluble organic selenium, and there is no relevant report of producing water-soluble organic selenium exceeding 3000 mg / kg in the prior art

Method used

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  • Bacillus subtilis SE201412 and organic-selenium-rich bactericide
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  • Bacillus subtilis SE201412 and organic-selenium-rich bactericide

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Effect test

Embodiment 1

[0063] The preparation method of embodiment 1 rich organic selenium bacterial agent

[0064] (1) Strain activation: add the rejuvenation medium into the test tube, put it under the gamma ray irradiation machine, after sterilizing under the condition of 2.5kGy, place it on an inclined plane, cool and solidify; Use an inoculation loop for strains to inoculate 1 loop on the test tube rejuvenation medium, inoculate 100 test tubes, and culture them in a 32°C incubator until the slant of the SE201412 test tube is covered with bacteria;

[0065] Wherein, the rejuvenation medium is: 6g glucose, 5g sucrose, 5g beef extract, 10 peptone, 4g sodium chloride, 18g agar, 1000ml water, pH7.4.

[0066] (2) Expanded cultivation: Take 100L of aerated tap water and put it into a 200L fermentation tank, then add 600g of glucose, 500g of sucrose, 500g of beef extract, 1000g of peptone, 400g of sodium chloride, and 0.1kg of GPE-6330 special defoamer for biological fermentation , adjust the pH to 7....

Embodiment 2

[0069] The preparation method of embodiment 2 rich organic selenium bacterial agent

[0070] (1) Strain activation: add the rejuvenation medium into the test tube, put it under the gamma ray irradiation machine, after sterilizing under the condition of 2.5kGy, place it on an inclined plane, cool and solidify; Use an inoculation loop for strains to inoculate 1 loop on the test tube rejuvenation medium, inoculate 200 test tubes, and culture them in a 32°C incubator until the slant of the SE201412 test tube is covered with bacteria;

[0071] Wherein, the rejuvenation medium is: 12g glucose, 10g sucrose, 10g beef extract, 20g peptone, 8g sodium chloride, 36g agar, 2000ml water, pH7.6.

[0072] (2) Expanded cultivation: Take 200L of aerated tap water and put it into a 0.5-ton fermentation tank, then add 1200g of glucose, 1000g of sucrose, 1000g of beef extract, 2000g of peptone, 800g of sodium chloride, and GPE-6330 special defoamer for biological fermentation 0.2 kg, adjust the p...

Embodiment 3

[0077] The preparation method of embodiment 3 rich organic selenium bacterial agent

[0078] (1) Strain activation: add the rejuvenation medium into the test tube, put it under the gamma ray irradiation machine, after sterilizing under the condition of 2.5kGy, place it on an inclined plane, cool and solidify; Use an inoculation loop for strains to inoculate 1 loop on the test tube rejuvenation medium, inoculate 50 test tubes, and culture in a 32°C incubator until the slant of the SE201412 test tube is covered with bacteria;

[0079] Wherein, the rejuvenation medium is: 3g glucose, 2.5g sucrose, 2.5g beef extract, 5 peptone, 2g sodium chloride, 9g agar, 500ml water, pH7.5.

[0080] (2) Expanded cultivation: Take 50L of aerated tap water and put it into a 100L fermentation tank, then add 300g of glucose, 250g of sucrose, 250g of beef extract, 500g of peptone, 200g of sodium chloride, and 0.05kg of GPE-6330 special defoamer for biological fermentation , adjust the pH to 7.6, use...

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Abstract

The invention relates to bacillus subtilis SE 201412 deposited under the accession number of CCTCC No. M 2015708. The strain has quite high selenium resistance, and can be fermented in a selenium-containing culture medium to generate fermentation liquor of which the organic selenium content reaches 5,000 mg / L-12,000 mg / L. The fermentation liquor is prepared into an organic-selenium-rich bactericide which is applied to leaf surfaces of crops in a growing period, selenium-rich agricultural products can be produced, the produced agricultural products can reach quality standard of organic-selenium-rich grain, oil, fruit, vegetables and dried fruit, the quality and the yield of the agricultural products can be improved, and disease resistance of the crops is improved.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to Bacillus subtilis SE201412 and organic selenium-rich bacterial agents. Background technique [0002] Selenium is an essential trace element for humans and animals. Selenium deficiency can cause more than 40 kinds of diseases. Current research shows that 50 micrograms of selenium per person per day can make people healthy and long-lived. It is considered the best way to supplement organic selenium through food. The safest and most convenient means. [0003] 75% of the countries and regions in the world are deficient in selenium and lack selenium. There are more than 20 selenium-deficient provinces and cities in my country, and the selenium content in the soil is about 0.03-0.1mg / kg. Supplementing selenium to the soil is a way to obtain selenium-rich agricultural products (food) effective way. Over the years, people have used raw materials such as selenium-enriched ore and seleni...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N59/02A01P21/00C05F11/08C12R1/125
CPCA01N59/02A01N63/00C05F11/08C12N1/205C12R2001/125A01N2300/00
Inventor 刘金龙郑威刘欋霄郑倬温亮戴光忠杨军孙志国
Owner HUBEI UNIV FOR NATITIES
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