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Artemisia japonica homogeneous polysaccharides as well as preparation method and application thereof

A technique of Artemisia annua and polysaccharide, applied in the field of Artemisia annua homogeneous polysaccharide and preparation thereof, can solve the problem of no extraction of homogeneous polysaccharide, little active effect of Artemisia annua polysaccharide, no research report on Artemisia univariate polysaccharide rheumatoid arthritis, etc. problem, to achieve the effect of reducing the degree of swelling of the foot and the preparation method is simple

Active Publication Date: 2016-09-21
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the polysaccharide components in Artemisia annua, and they mainly focus on the extraction and separation methods of polysaccharides. The extracted polysaccharides are mixed polysaccharides, and there are few research reports on the activity of polysaccharides in Artemisia annua.
In particular, there is no research report on the extraction of homogeneous polysaccharides from Artemisia annua, and there is no research report on the use of homogeneous polysaccharides in the treatment of rheumatoid arthritis.

Method used

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  • Artemisia japonica homogeneous polysaccharides as well as preparation method and application thereof
  • Artemisia japonica homogeneous polysaccharides as well as preparation method and application thereof
  • Artemisia japonica homogeneous polysaccharides as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Preparation and identification of the homogeneous polysaccharide of Artemisia annua

[0038] 1.1 Preparation of homogeneous polysaccharide from Artemisia annua

[0039] a) Take 2 kg of dried Artemisia annua shredded, add 20 L of ethanol with a mass fraction of 75% to reflux degrease (to remove low-polarity components), reflux degrease after 10 hours, filter to obtain an ethanol-insoluble residue, and dry Residue, then add boiling water, reflux extraction twice, each time 4h, the ratio of solid to liquid for the first extraction is 1:12 (ie: 1Kg residue, use 12L of water), the ratio of solid to liquid for the second extraction is 1:10 (i.e.: 1Kg residue, use 10L water); filter, combine two extracts, concentrate, add ethanol with a mass fraction of 95%, stir while adding, until the mass fraction of final ethanol is 75%, let it stand for alcohol precipitation for 24 hour, filter, collect the precipitate and wave off the residual ethanol; centrifuge the precipit...

Embodiment 2

[0064] Example 2: In vivo pharmacodynamic experiments in mice with arthritis

[0065] 1) Establishment of collagen-induced arthritis mouse model:

[0066] Animals: 6-8 weeks old, 12 female DBA / 1 mice, bred in the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine.

[0067] Reagents: bovine type II collagen (CII), complete Freund's adjuvant (CFA), and incomplete Freund's adjuvant (IFA) were purchased from Chondrex, USA.

[0068] Methods: For the first immunization, 100 μL of CFA emulsion containing 100 μg CⅡ was injected intradermally at the base of the tail of DBA / 1 mice, which was recorded as the first day, and a booster immunization was performed on the 22nd day, and CFA containing 100 μg CⅡ was injected intradermally. The emulsion of IFA was 100 μL, and the clinical manifestations of the mice were observed.

[0069] Scoring: scoring every two days by two independent observers; scoring criteria: 0, no erythema and edema; 1, erythema and mild ...

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Abstract

The invention discloses Artemisia japonica homogeneous polysaccharides as well as a preparation method and an application thereof. The Artemisia japonica homogeneous polysaccharides are obtained through column chromatographic separation and purification after water extraction and alcohol precipitation of all grass of Artemisia japonica, are inulin type fructan which is connected by fructose through 1,2 and contains a glucosyl group at the terminal, contain no uronic acid and have the weight-average molecular weight ranging from 1 kDa to 20 kDa. Experiment results indicate that the Artemisia japonica homogeneous polysaccharides can obviously reduce the foot swelling degree of mice suffering from collagen-induced arthritis and levels of inflammatory factors IL-6 and IL-17A in serum of the mice, are expected to be used as a major or unique active ingredient for preparation of health-care food or pharmaceutic preparations for preventing or / and treating rheumatoid arthritis, and have the important significance in research and development of Artemisia japonica pharmaceutic preparations having the clinical application value; besides, the preparation method is simple, high-purity Artemisia japonica homogeneous polysaccharides can be obtained, and the method is suitable for large-scale production and good in practicability.

Description

technical field [0001] The invention relates to a homogeneous polysaccharide from Artemisia annua and its preparation method and application, belonging to the technical field of natural medicines. Background technique [0002] Rheumatoid arthritis (RA) is a common autoimmune disease. If it is not treated in time, the late disability rate will be as high as 70%. It is reported that the number of patients with rheumatoid arthritis in my country has exceeded 5 million. The drugs commonly used clinically to treat RA are mainly western medicines, such as methotrexate, sulfasalazine, and inflammatory factor monoclonal antibodies, etc. Although the effect is quick, the side effects are also relatively obvious. At present, finding therapeutic drugs for RA from traditional Chinese medicine has become a research hotspot. [0003] Artemisia annua is the dried whole herb of Artemisia genus Artemisia in the family Asteraceae. It tastes bitter, slightly sweet, and cool in nature. Yuecai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/733A61P19/02A61P29/00
CPCA61K31/733C08B37/0003C08B37/0051
Inventor 王顺春李宁施松善施晨晨苏娟刘睿敏
Owner SHANGHAI UNIV OF T C M
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