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Method for producing compound feed from mixed strains through united fermentation

A technology of compound feed and joint fermentation, which is applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the economic losses of the country and enterprises, the return and destruction of livestock products, etc., to enhance disease resistance, reduce loss, reduce The effect of pH

Inactive Publication Date: 2016-09-21
INNER MONGOLIA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, every year in my country, livestock products are returned or destroyed due to excessive drug residues, which has caused huge economic losses to the country and enterprises.

Method used

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  • Method for producing compound feed from mixed strains through united fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Pretreatment of corn bran: The corn bran is mechanically crushed and passed through a 40-mesh sieve, and then pretreated by high temperature and high pressure method.

[0028] 2. Preparation of beneficial fermenting agent: the required fermenting strains of Trichoderma and Aspergillus niger were cultured on inclined planes, shake flasks and koji, respectively, and then mixed according to the volume ratio of 30:70 to make a first-grade solid starter; The required strains of Saccharomyces cerevisiae, Pichia pastoris and Bacillus subtilis were respectively cultured in test tubes, shake flasks and seed tanks, and then the cultured bacteria liquids were respectively inserted into the liquid medium according to the inoculation amount of 5%~10%. After 2 days of culture, it becomes a mature liquid, and then according to the volume ratio, Saccharomyces cerevisiae: Pichia pastoris: Bacillus subtilis = 20:20:60 is formulated into a secondary solid-state starter; After the koji ...

Embodiment 2

[0034]1. Pretreatment of corn bran: The corn bran is mechanically crushed and passed through a 40-mesh sieve, and then pretreated by ultrasonic method.

[0035] 2. Preparation of beneficial fermenting agent: the required fermenting strains Trichoderma and Aspergillus niger were cultured on inclined planes, shake flasks, and koji, respectively, and then mixed according to the volume ratio of 40:60 to make a first-grade solid starter; The required strains of Saccharomyces cerevisiae, Pichia pastoris and Bacillus subtilis were respectively cultured in test tubes, shake flasks and seed tanks, and then the cultured bacteria liquids were respectively inserted into the liquid medium according to the inoculation amount of 5%~10%. After 2 days of culture, it becomes a mature liquid, and then according to the volume ratio, Saccharomyces cerevisiae: Pichia pastoris: Bacillus subtilis = 20:30:50 is formulated into a secondary solid-state starter; After the koji culture, the three-stage so...

Embodiment 3

[0041] 1. Pretreatment of corn bran: The corn bran is mechanically crushed and passed through a 40-mesh sieve, and then pretreated by high temperature and normal pressure method.

[0042] 2. Preparation of beneficial fermenting agent: the required fermenting strains of Trichoderma and Aspergillus niger are cultured on inclined planes, shake flasks and koji, respectively, and then mixed according to the volume ratio of 50:50 to make a first-grade solid starter; The required strains of Saccharomyces cerevisiae, Pichia pastoris and Bacillus subtilis were respectively cultured in test tubes, shake flasks and seed tanks, and then the cultured bacteria liquids were respectively inserted into the liquid medium according to the inoculation amount of 5%~10%. After 2 days of culture, it becomes a mature liquid, and then according to the volume ratio, Saccharomyces cerevisiae: Pichia pastoris: Bacillus subtilis = 30:20:50 is formulated into a secondary solid-state starter; the required La...

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Abstract

The invention relates to a method for producing compound feed from mixed strains through united fermentation. According to the method, corn bran is taken as a main raw material and is subjected to batch culture through Aspergillus niger, trichoderma, bacillus subtilis, saccharomycetes and lactic acid bacteria, the microbiological feed is produced through combined conversion and solid fermentation, and the method comprises technological steps as follows: pretreatment of the raw materials, compounding of a mould starter, blending of yeast composite fermentation preparation, preparation of lactic acid bacteria starter, separate solid fermentation, drying, smashing and the like. According to the invention, the corn bran resource can be utilized sufficiently and efficiently, and the additional value of the corn bran resource is increased; harmful bacterial colonies in intestinal tracts can be reduced, the functions of conditioning healthcare, increasing the feed utilization rate, preventing diseases, promoting growth, reducing usage of drugs such as antibiotics and the like and improving the quality of livestock and poultry products are effectively performed, excrement stink is reduced, the breeding environment is improved, and one high-quality and low-cost green biological feed for feeding livestock and poultry is provided.

Description

technical field [0001] The invention relates to a method for producing compound feed through joint fermentation of mixed strains, and belongs to the technical field of biological feed fermentation production. Background technique [0002] In recent years, feed resource constraints have gradually become a bottleneck in the development of China's feed industry and even animal husbandry production. At present, China's feed grain accounts for about 35% of the total grain output, and it is estimated that by 2020 and 2030, the proportion will reach 45% and 50% respectively, but the expected annual increase of grain is only about 1%, and the gap of feed grain is inevitable. High-quality protein feed resources will be more tense. Therefore, trying to use new feed ingredients to replace the increasingly scarce conventional feed ingredients will become an inevitable trend in the development of feed in the future. Among them, some by-products of grain deep processing will be a resear...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/30A23K10/37A23K20/20A23K20/26
CPCY02P60/87
Inventor 刘占英刘艳新李永丽石雅丽胡建华倪慧娟
Owner INNER MONGOLIA UNIV OF TECH
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