Efficient preparing method for high-purity flaxseed protein

Abstract

The invention discloses an efficient preparing method for high-purity flaxseed protein. The efficient preparing method comprises the following steps that firstly, defatted flaxseed meal is taken, dried and ground, and flaxseed meal powder is obtained; secondly, the flaxseed meal powder is taken and evenly mixed with 10-30 folds (v / m) of a NaCl solution, and extracting is carried out; thirdly, a flaxseed powder extracting solution is centrifuged, residues are removed, and supernatant is obtained and is a crude flaxseed protein extracting solution; fourthly, the concentration of NaCl of the crude flaxseed protein extracting solution is regulated to be 0.02-0.05 mol / L, then the crude flaxseed protein extracting solution is added into finely-mixed zwitterion exchange resin, and stirring is carried out until the change range of the pH value is smaller than 0.2; fifthly, a NaOH solution with the pH being 9-11 is used for washing the zwitterion exchange resin; sixthly, a NaCl solution with the pH being 4-6 is used for washing the zwitterion exchange resin, and a flaxseed protein extracting solution is obtained; seventhly, drying is carried out, and the high-purity flaxseed protein is obtained. By means of the method, flaxseed protein can be effectively extracted, the extracting rate is high, the technological steps are simple, cost is low, and the prepared flaxseed protein is very high in purity and good in effect.

Description

technical field [0001] The invention relates to a high-efficiency preparation method of high-purity flaxseed protein. Background technique [0002] Flaxseed is the dry mature seed of Linum usitatissimum L., a plant of the family Linaceae, also known as flaxseed and linseed. for wild. Flaxseed is an important plant resource for both medicine and food. It is recorded in the "Chinese Pharmacopoeia". Modern pharmacological studies have shown that flaxseed has a variety of biological activities, including anti-tumor effects, antioxidant effects, immunosuppressive effects, and hypoglycemic and blood lipid-lowering effects. Flaxseed is flat oval, 4-7mm long, 2.5mm wide, 1.5mm thick, and 1,000-grain weight is 3.5-11g. Flaxseed consists of a skin, husk and cotyledons. The epidermis is 0.1-0.2mm thick, mainly composed of carbohydrate complex compounds such as starch. The cotyledons account for slightly more than half of the seed weight and contain most of the oil and protein. Th...

AI Technical Summary

Problems solved by technology

When using defatted flaxseed as raw material to extract flax protein isolate, because flaxseed is rich in colloid, which interferes with the separation and precipitation of protein, the extraction is very difficult. The yield is about 42%, and the purity of the protein reaches 89.37%. However, flaxseed needs a lot of energy to dry after degumming and before degreasing. The production cost of each product of this process is relatively high, so it has not been applied to industrial production so far.

[0005] Xu Guangying, etc., "Research on the Extraction Process of Flax Protein Isolate" Chinese Journal of Cereals and Oils, Volume 28, Issue 3, March 2013, disclosed an improved method of extracting flaxseed protein from defatted flaxseed, but the yield is still very low , only 52.17%