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Flaxseed peptides having effects of lowering cholesterol and preparation method thereof

A technology for lowering cholesterol and flaxseed is applied in the field of food-derived protein deep processing, which can solve the problems of restricting the application of flaxseed meal, and achieve the effect of reducing loss

Active Publication Date: 2016-09-07
瑞泰高直生物科技(武汉)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the human body eats flaxseed meal containing cyanogenic glycosides, 10-20% of the cyanogenic glycosides can be decomposed into toxic hydrocyanic acid through the action of intestinal microorganisms, which severely limits the application of flaxseed meal
At present, there is no method of detoxification by low-temperature weak acid water washing, adding β-glucosidase and α-cyanohydrinase to decompose cyanogenic glycosides into volatile hydrocyanic acid, and then completely remove cyanogenic glucosides and hydrocyanic acid toxins. to report

Method used

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  • Flaxseed peptides having effects of lowering cholesterol and preparation method thereof
  • Flaxseed peptides having effects of lowering cholesterol and preparation method thereof
  • Flaxseed peptides having effects of lowering cholesterol and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0018] The preparation of embodiment 1 lowering cholesterol flaxseed peptide

[0019] After cleaning the flax meal to remove impurities, add a solution containing 0.1% citric acid and 0.1% glacial acetic acid according to the material-to-water ratio of 1:5 (w / v), wash with water at a controlled temperature of 20°C for 0.5h, drain and dry according to the ratio of material to liquid 1:10 (w / v) add water and beat. Adjust the pH of the beating solution to 8.0, add pectin lyase with a substrate concentration of 1%, and perform enzymatic hydrolysis at 30°C for 1 hour. After enzymatic hydrolysis and degumming, adjust the pH to 7.5, and sequentially add papain, 2% neutral protease, 1% β-glucosidase, and 1% α-hydroxyhydrinase at the substrate concentration for 2 hours. . Heat the enzymolysis solution to boiling and keep it for 1 hour, turn on the ultrasonic wave 5 minutes before the end of the enzyme inactivation, the frequency is 30KHZ and the ultrasonic wave is continuous for 5 mi...

Embodiment 2

[0020] The preparation of embodiment 2 lowering cholesterol flaxseed peptide

[0021] After cleaning the flax meal to remove impurities, add a solution containing 0.2% citric acid and 0.5% glacial acetic acid according to the material-to-water ratio of 1:20 (w / v), wash it with water at a controlled temperature of 25°C for 1.5 hours, drain it, and mix it according to the material-liquid ratio 1:20 (w / v) add water and beat. Adjust the pH of the beating solution to 9.5, add pectin lyase with a substrate concentration of 1.5%, and perform enzymatic hydrolysis at 32°C for 2 hours. After enzymatic hydrolysis and degumming, adjust the pH to 7.0, and add papain with substrate concentration of 2%, neutral protease with 2%, β-glucosidase with 1.5%, and α-hydroxyhydrinase with 1.5% for 3 hours. . Heat the enzymolysis solution to boiling and keep it for 2 hours, turn on the ultrasound 8 minutes before the end of the enzyme inactivation, the frequency is 40KHZ continuous ultrasound for 8...

Embodiment 3

[0022] Embodiment 3 Preparation of cholesterol-lowering flaxseed peptide

[0023] After cleaning the flax meal to remove impurities, add a solution containing 0.2% citric acid and 0.2% glacial acetic acid according to the material-to-water ratio of 1:10 (w / v), wash it with water at a controlled temperature of 40°C for 1 hour, drain it, and use a material-to-liquid ratio of 1 :12(w / v) add water and beat. Adjust the pH of the beating solution to 9.3, add pectin lyase with a substrate concentration of 0.8%, and perform enzymatic hydrolysis at 26°C for 2 hours. After enzymatic hydrolysis and degumming, adjust the pH to 6.5, and sequentially add papain, 1% neutral protease, 1% β-glucosidase, 1% α-hydroxyhydrinase with a substrate concentration of 1.5 h. Heat the enzymolysis solution to boiling and keep it for 0.5h, turn on the ultrasonic wave 3min before the end of the enzyme inactivation, the frequency is 25KHZ and the ultrasonic wave is continuous for 3min. After the enzyme in...

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Abstract

The present invention discloses flaxseed peptides having effects of lowering cholesterol and a preparation method thereof. The preparation method includes the following steps: flaxseed selecting and impurity removing, primary low-temperature and weak-acid water washing, primary beating, primary pectin lyase enzymatic digesting and degumming, primary papain, neutral protease, beta-glucosidase, alpha-cyanohydrin protein and hydroxynitrile lyases protein hydrolysate and cyanogenic glycoside decomposition reacting, ultrasonic high-temperature enzyme inactivating and detoxifying, and then centrifuging, nano-filtering, spray-drying, and cholesterol-lowering flaxseed peptide obtaining. The preparation method solves the effects of the flaxseed gum on the flaxseed proteolysis and removes cyanogenic glycosides and hydrocyanic acids in the products of the flaxseed peptides. The flaxseed peptide products are prepared for the first time, can be applied in the food and health-care food industries, and have broad business outlooks.

Description

technical field [0001] The invention belongs to the technical field of food-derived protein deep processing, and relates to a method for preparing linseed peptide with cholesterol-lowering activity by using linseed meal as a raw material. Background technique [0002] Flax (also known as flax) is an ancient economic crop and one of the top ten oil crops in the world, widely distributed all over the world. China's flaxseed production ranks second in the world, with an output of about 400,000 tons in 2013. Flaxseed contains fatty acids, protein, flaxseed gum, lignans, minerals, vitamins and other ingredients with nutritional value and functions. In addition to the linseed oil that has been developed and utilized traditionally, the linseed meal, the remaining product after refining linseed oil, is basically used as fertilizer or added to animal feed in limited quantities, and its potential value is far from being exploited. Flax meal contains 30-40% protein, about 10-15% lins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/14A23J3/34
Inventor 陈栋梁莫朝晖陈飞盛彩虹
Owner 瑞泰高直生物科技(武汉)有限公司
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