A kind of Huolinan tissue culture rapid propagation seedling raising method

Abstract

The invention relates to a michelia macclurei tissue culture fast propagation seedling culture method, which comprises the following steps of seed sterilization, cluster bud induction, successive transfer culture, rooting culture, domestication culture and rooting seedling transplanting, wherein a cluster bud induction culture medium, a successive basic culture medium and a rooting basic culture medium are all improved. Through the technical methods of regulating the mixture ratio of five major elements, regulating trace element concentrations, adding antioxidants and the like, the problems of poor seedling growth or even vitrification or browning death due to unbalanced nutrition elements of partial embryonic system seedlings at 0.7MS can be solved; healthy and strong seedlings with the height being 1.5 to 4.0cm are produced; high-quality successive seedling culture is realized; the adaption to the use of most embryos is realized; the strong seedling culture link is reduced; the cost is reduced; meanwhile, the efficient industrialized production is realized; the culture cost is reduced; the management difficulty is reduced; the browning death of the seedlings due to too long culture time is avoided; the transplanting survival rate reaches 90 percent or higher.

Description

technical field [0001] The invention relates to the technical field of breeding of fine varieties of tropical forest trees and seedling propagation, in particular to a method for rapid propagation and seedling raising of Huolinan tissue culture. Background technique [0002] Micheliamacclurei Dandy is an evergreen broad-leaved tree belonging to the family Dagnoliaceae. It is distributed in Guangdong, Hainan, northern Guangxi and northern Vietnam. It grows fast and has strong adaptability. The main tree species for afforestation and excellent soil improvement tree species. The trunk is straight, the cut surface of the wood is smooth and beautiful, the hardness is high, it is resistant to decay, easy to dry, fragrant, less cracked, and not warped. It is an excellent furniture and building material. Its flowers are gorgeous, the tree shape is beautiful, and the forest stands are poor in combustion. It is an excellent woody flower, garden landscape tree and street tree. [000...

AI Technical Summary

Problems solved by technology

This culture method all can obtain higher survival rate, but also has the weak point, its adventitious bud induction, cluster bud induction, rooting culture all need to pass through dark culture, and use peptone, coconut milk, its rooting culture time is 30 -Rooting within 45 days, training time for seedlings is 30 days, there are the following deficiencies: 1, the operation links are many and complicated and the cost of peptone and coconut milk cultivation is high

2. The time for rooting and seedling training is too long. The 60-75 days of rooting and seedling training will not only greatly increase the cost of cultivation, but also increase the pollution rate and reduce production efficiency; the long-term rooting and training of seedlings will increase management. , for the Huolinan sprouts that are easy to brown, they are not only prone to browning and death, but also prone to death due to nutrient depletion

There are the following deficiencies: there are defects in the proliferation medium, resulting in long seedling transfer cycle, low height growth, low multiplication factor, low effective seedlings, and the need for strong seedlings to take root and cultivate, which is not conducive to profitable industrial production

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