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A kind of method utilizing blueberry tissue culture seedling to carry out cutting propagation

A technology of cutting propagation and tissue culture seedlings, which is applied in horticultural methods, vegetative propagation, botanical equipment and methods, etc., can solve the problems of low rooting rate, achieve the effects of long time, reduce tissue culture links, and high cost

Active Publication Date: 2020-07-31
黑龙江朴厚农林科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with tissue culture, cutting propagation is widely used in plant seedling breeding because of its simplicity, short seedling cultivation cycle, and fast rooting speed. However, conventional cutting propagation methods are limited by the number of cuttings and the time for obtaining materials. The low rooting rate of blueberry direct cuttings is the bottleneck restricting the development of clonal production using blueberry cuttings

Method used

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  • A kind of method utilizing blueberry tissue culture seedling to carry out cutting propagation
  • A kind of method utilizing blueberry tissue culture seedling to carry out cutting propagation
  • A kind of method utilizing blueberry tissue culture seedling to carry out cutting propagation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Establishment of cutting propagation method of blueberry tissue culture seedlings

[0034] 1Materials and methods

[0035] 1.1 The cultivation of blueberry tissue culture seedlings

[0036] 1.1.1 Materials and preliminary treatment

[0037] The experiment was carried out in the Laboratory of Horticulture Branch of Heilongjiang Academy of Agricultural Sciences from November 2014 to September 2016. The test materials were collected in the blueberry garden of the Horticulture Branch of the Heilongjiang Academy of Agricultural Sciences in November 2014. The blueberry varieties were Berkeley, Mayden and Sunrise.

[0038] Bring the one-year shoots of the three species collected to the laboratory, and use hydroponics to promote the axillary buds to germinate. When the axillary buds germinate and grow to about 2cm, they are cut off, placed in a clean bench, and soaked in 75v / v% alcohol 30s, rinse with sterile water 4 times and then transfer to 0.1w / w%HgCl 2 Disinfect it for 8...

Embodiment 2

[0108] Example 2 Morphological and anatomical study of blueberry cuttings during rooting

[0109] Taking blueberry maytan tissue culture seedlings as experimental materials, the changes of external morphology and rooting anatomy during the development of adventitious roots of cuttings were studied.

[0110] 1 Cutting propagation method of blueberry Maytan tissue culture seedlings

[0111] Using the optimal technical solution established in Example 1, specifically includes the following steps:

[0112] (1) The collected 1-year-old branches of Blueberry Mayden are hydroponics to promote the axillary buds to germinate. When the axillary buds germinate and grow to 2cm, they are cut off, soaked in 75v / v% alcohol for 30s, rinsed with sterile water 4 times and then transferred to 0.1 w / w%HgCl 2 Sterilize in the solution for 8 minutes, and finally rinse with double distilled water for 5 times, put it into the ultra-clean workbench after disinfection, and receive it into the primary culture me...

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Abstract

The invention discloses a method for cutting propagation by utilizing blueberry tissue culture seedlings. The method disclosed by the invention utilizes the blueberry tissue culture seedlings as cuttings for cutting propagation, combines a rooting culture stage of subculture sprouts in a bottle with transplanting domestication of rooting seedlings, reduces a tissue culture step, saves production costs and greatly shortens rooting time, thereby shortening a seedling raising cycle; and the method explores conditions affecting rooting of cutting of the blueberry tissue culture seedlings through researching influences of different conditions on the rooting of the cutting of the blueberry tissue culture seedlings, thereby improving a rooting rate by utilizing artificial regulation and control.The invention establishes a novel method capable of rapidly reproducing the blueberry seedlings in quantity, and the method provided by the invention can meet heavy demand for blueberries by markets.

Description

Technical field [0001] The invention relates to a blueberry tissue culture method, in particular to a method for cutting propagation using blueberry tissue culture seedlings. The invention belongs to the technical field of agricultural planting. Background technique [0002] Blueberry (blueberry), a plant belonging to the genus Vaccinium of the Eri-caceae family (Eri-caceae), also known as bilberry, blue berry, is a small perennial berry shrub fruit tree. [0003] Blueberry fruits are rich in sugar, acid, protein, vitamins, and mineral elements. In addition, they also contain anthocyanins, arbutin, niacin, tannic acid, flavonoids, antioxidants, folic acid and other substances. The fruit has health-care functions and medicinal values ​​such as protecting eyesight, relieving visual fatigue, delaying aging, anti-cancer, enhancing human immunity, and softening blood vessels. In addition, the blueberry fruit has extremely high economic value. In addition to being eaten fresh, the fruit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G2/10A01H4/00A01G24/22A01G24/15C05G1/00C05G5/20
CPCA01H4/001A01H4/008C05D9/00C05D9/02C05G3/00C05G5/23C05F11/00C05F11/02
Inventor 杨瑞华闫绍鹏王柏林鲁会玲覃杨叶万军王禹董畅任爱华刘万达肖丽珍王雪娇
Owner 黑龙江朴厚农林科技有限公司
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