Specific detection and quantification of cardiolipin and isolated mitochondria by positively charged AIE fluorogens and method of manufacturing of AIE fluorogens

Abstract

The present subject matter relates to a one-step method of detecting and quantifying cardiolipin in a sample using a positively charged AIE luminogen by introducing the AIE luminogen to a solution containing the sample and measuring fluorescence intensity of the solution; a method of quantifying isolated mitochondria using a positively charged AIE luminogen by staining a sample containing isolated mitochondria with the AIE luminogen and measuring the fluorescence intensity; and a method of quantifying isolated mitochondria using a positively charged AIE luminogen by introducing the AIE luminogen to a sample containing isolated mitochondria, wherein the AIE luminogen stains the isolated mitochondria and identifying the stained isolated mitochondria under microscope. With improved sensitivity and excellent selectivity to CL over other major mitochondrial membrane lipids, an aggregation-induced emission-active fluorogen, TTAPE-Me, may serve as a valuable fluorescent sensor for CL detection and quantification and the quantification of isolated mitochondria.

Description

[0001] Cross References to Related Applications [0002] This patent application claims priority to U.S. Provisional Patent Application No. 61 / 963,393, filed December 3, 2013, the applicant of which is the inventor of this patent application, the entire contents of which are incorporated herein by reference . technical field [0003] The subject of the present invention relates in particular to compounds or salts which allow the quantification of cardiolipin (CL) and which allow the quantification and identification of isolated mitochondria, in particular from the yeast S. cerevisiae strain YPH 500. Background technique [0004] Eukaryotic cells use about 5% of their genes to synthesize lipids. Due to the indispensable functions of lipids in cells, a large fraction of lipids is utilized. Due to their unique structure, lipids form bilayer structures to separate internal components from the extracellular environment and to compartmentalize individual cellular organelles. In...

AI Technical Summary

Problems solved by technology

In addition, the small Stokes shift and poor water solubility of NAO make it difficult to use in biological systems

How NAO works is unclear and performance is difficult to improve, even with various processing methods

Although NAO has many disadvantages and even does not have a standard experimental protocol, it has been used for many years because there is no other alternative

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