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Metabolism marker group used for diagnosing and differentiating stable angina pectoris and acute coronary syndrome

A technology for stable angina pectoris and metabolic markers, which is applied in the field of biochemistry to achieve the effect of promoting standardization, improving diagnostic convenience and high sensitivity

Inactive Publication Date: 2016-08-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] No one has used plasma metabolite levels to diagnose coronary heart disease

Method used

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  • Metabolism marker group used for diagnosing and differentiating stable angina pectoris and acute coronary syndrome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening Characterization of Differential Metabolites in Plasma Between Patients with Stable Angina and Acute Coronary Syndrome

[0026] 1. Objects and methods

[0027] 1. The source of the specimen

[0028] After obtaining the consent of the patients, the peripheral venous blood plasma of 280 patients with stable angina pectoris, 320 patients with acute coronary syndrome and 350 healthy people in Jiangsu Provincial People's Hospital from September 2010 to June 2015 were collected. Confirmed by coronary angiography. The age and gender of healthy people were matched with patients with stable angina pectoris and patients with acute coronary syndrome. All patients with stable angina pectoris, patients with acute coronary syndrome and healthy people had normal heart, lung, liver, kidney and hematopoietic functions.

[0029] The time of blood collection was in the morning on an empty stomach.

[0030] 2. Main reagents

[0031] Acetonitrile and formic acid (U...

Embodiment 2

[0052] Example 2: Construction of ROC curve to verify the ability of 6 differential metabolites to diagnose stable angina pectoris and acute coronary syndrome

[0053] Receiver operating curve (ROC) method was used for verification, and the expression levels of differential metabolites in the plasma of patients with stable angina pectoris and patients with acute coronary syndrome were used to diagnose and distinguish patients with stable angina pectoris and patients with acute coronary syndrome Ability. The results showed that six differential metabolites, malic acid, taurine, arachidonic acid, citramalic acid, methionine, and pentadecanoic acid, were individually used to diagnose patients with stable angina pectoris and patients with acute coronary syndrome The ability is strong, and the area under the ROC curve (AUC) is greater than 0.7, which has clinical diagnostic significance; when combined for diagnosis, with the increase of the number of combinations, the AUC further i...

Embodiment 3

[0062] Embodiment 3: the preparation of detection kit

[0063] A detection kit has been prepared based on the metabolic markers provided by the present invention, and the kit includes the following components:

[0064] Standard products of metabolic markers: including malic acid, taurine, arachidonic acid, citramalic acid, methionine, pentadecanoic acid, each standard product is packaged separately;

[0065] Plasma metabolite extraction solvent: 100% acetonitrile and 20% acetonitrile aqueous solution (for UPLC-Q / TOF-MS sample preparation); a mixed solution of methanol, chloroform and water in a ratio of 2.5:1:1, methoxyaminopyridine and N-Methyl-N-trimethylsilyl trifluoroacetamide (for GC-Q / MS sample preparation); 20% acetonitrile aqueous solution can be used as dissolved standard in UPLC-Q / TOF-MS screening and characterization Solvent; in GC-Q / MS screening and characterization, use the plasma metabolite extraction solvent to prepare the standard solution according to the sam...

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Abstract

The invention discloses a metabolism marker group used for diagnosing and differentiating stable angina pectoris and acute coronary syndrome. The metabolism marker group comprises one or more selected from a group consisting of malic acid, taurine, arachidonic acid, citramalic acid, methionine and pentadecanoic acid. When an individual metabolism marker is used for diagnosing and differentiating patients with stable angina pectoris and patients with acute coronary syndrome, an area under a ROC curve (AUC) is greater than 0.7, the individual metabolism marker has clinical diagnosis significance; when metabolism markers are combined and used for diagnosis, AUC further increases with increase of the number of the combined metabolism markers; and AUC reaches a highest value of 0.987 when all the six metabolism markers are combined, and sensitivity and specificity are 96.8% and 97.7% respectively under the condition of an optimal cutoff value. The metabolism marker group provided by the invention can accurately diagnose and differentiate stable angina pectoris and acute coronary syndrome and has high sensitivity and good specificity.

Description

technical field [0001] The invention belongs to the field of biochemistry, and relates to metabolic markers for diagnosis and classification of coronary heart disease, in particular to a group of metabolic markers for diagnosing and distinguishing stable angina pectoris and acute coronary syndrome. Background technique [0002] Coronary heart disease, also known as ischemic heart disease, involves atherosclerosis in the arteries supplying myocardial blood, that is, coronary atherosclerotic lesions cause vascular lumen narrowing or plaque formation or even rupture and complete blockage, resulting in myocardial ischemia and hypoxia. Or necrosis leads to cardiac ischemic disease, which leads to a series of serious cardiovascular events such as clinical angina pectoris and myocardial infarction. Coronary heart disease is the main killer of human health. It has the characteristics of high incidence rate, high disability rate, high recurrence rate, high mortality rate, and many co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027G01N2030/025G01N30/72G01N33/6893G01N2800/324G01N2570/00G01N30/7206G01N30/7233G01N33/492G01N2560/00
Inventor 齐炼文范勇李萍朱伟陈彦
Owner CHINA PHARM UNIV
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