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Method for rapidly measuring content of five saponins in radix-notoginseng medicinal materials with near infrared spectroscopy method and application

A near-infrared spectroscopy and near-infrared spectroscopy technology, which is applied in the field of traditional Chinese medicine research, can solve time-consuming and labor-intensive problems, achieve efficient technical methods, save manpower and material resources, and shorten test time.

Inactive Publication Date: 2016-08-17
HARBIN ZHENBAO PHARMA +1
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Problems solved by technology

The existing methods for determining the content of saponins in Panax notoginseng are mainly high-performance liquid phase analysis methods, which are time-consuming and labor-intensive, and require reference substances

Method used

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  • Method for rapidly measuring content of five saponins in radix-notoginseng medicinal materials with near infrared spectroscopy method and application
  • Method for rapidly measuring content of five saponins in radix-notoginseng medicinal materials with near infrared spectroscopy method and application
  • Method for rapidly measuring content of five saponins in radix-notoginseng medicinal materials with near infrared spectroscopy method and application

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Effect test

experiment example 1

[0043] 1 Sample collection: 173 groups of different parts of Panax notoginseng (including notches, main roots, tendons, fibrous roots, etc.), different origins (including Wenshan Prefecture, Honghe Prefecture, Kunming City, Yuxi City, etc.), different sizes and specifications The notoginseng samples were vacuum-dried, crushed and passed through a 280 μm sieve.

[0044] 2 methods

[0045] 2.1 Determination of the contents of five medicinal saponins in samples by HPLC

[0046] 2.1.1 Preparation of the test solution: Accurately weigh 0.5 g of Panax notoginseng powder, extract with 70% methanol water (v / v) 40 mL, and ultrasonically (working frequency 53 kHz) for 1 hour. Filter and wash the residue well with 70% methanol to allow complete transfer. Rotary evaporate to dryness at 60°C. Transfer to a 5.0mL volumetric flask with 70% methanol, and dilute to volume with the same solvent. Pass through a 0.22μm nylon filter membrane to obtain it.

[0047] 2.1.2 Chromatographic condit...

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Abstract

The invention relates to a method for rapidly measuring the content of five saponins in radix-notoginseng medicinal materials with the near infrared spectroscopy method and an application. The method includes the steps that different-batch radix notoginseng medicine materials are collected to compose a sample set, near infrared spectroscopy of the materials is collected, abnormal samples are eliminated, a calibration set and a verification set are divided, the suitable spectrum pretreatment method and suitable modeling wave bands are selected, the content of five-medicine-effect saponins in the radix-notoginseng medicinal materials is measured with the HPLC method to serve as the reference value, the chemometrics technology is applied, and a quantitative calibration model between the near infrared spectroscopy of the radix-notoginseng medicine materials and the content of the five-medicine-effect saponins of the radix-notoginseng medicine materials is built, and can be used for measuring the content of five medicine-effect saponins of unknown-content radix-notoginseng medicine materials and relative preparation production of the radix-notoginseng medicine materials. According to the method, the five medicine-effect saponins are main medicine-effect ingredients of a radix-notoginseng saponin preparation, and are similar to the quality control index of the five medicine-effect saponins; in this way, the rapid and efficient technology method can be provided for the quality control over the radix-notoginseng medicinal materials and the preparation of the radix-notoginseng medicinal materials, and the checking workload is reduced.

Description

technical field [0001] The invention relates to a method for rapidly determining the contents of five kinds of saponins in medicinal materials of Panax notoginseng, in particular to a method for rapidly determining the contents of five medicinal saponins in medicinal materials of notoginseng by using near-infrared spectroscopy, and belongs to the technical field of traditional Chinese medicine research. Background technique [0002] Panax notoginseng is the dried root and rhizome of Panax notoginseng (Burk) F.H.Chen, an Araliaceae plant. It has the effects of dispelling blood stasis, stopping bleeding, reducing swelling and relieving pain. It is an important common Chinese medicine and has been used in China for hundreds of years. . Among them, the saponins of Panax notoginseng are considered to be effective substances in traditional Chinese medicine. The injection preparations of traditional Chinese medicine made of total saponins of Panax notoginseng, such as Xuesaitong an...

Claims

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Application Information

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IPC IPC(8): G01N21/359
CPCG01N21/359
Inventor 方同华程翼宇瞿海斌杨振中许照芹席桂才张永年王春生孙海峰孙淑娜季庆亮徐秀杰周广红贾文娟王维龙
Owner HARBIN ZHENBAO PHARMA
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