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Novel preparation for mobilization of bone marrow hematopoietic stem cells

A technology of hematopoietic stem cells and bone marrow hematopoiesis, which is applied in the direction of animal cells, vertebrate cells, cell culture active agents, etc., and can solve the problem of lack of preparations or methods for mobilizing bone marrow hematopoietic stem cells, lack of G-CSF mobilization preparations, lack of hematopoietic stem cell mobilization methods, etc. problem, to achieve the effect of short mobilization cycle

Inactive Publication Date: 2016-08-17
GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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Problems solved by technology

[0007] Although according to literature reports and our previous research results, it is known that the human cell assembly-like stimulating factor G-CSF plays a key role in the stimulation of hematopoietic stem cells, but there is still a lack of an effective G-CSF mobilization preparation, and there is still a lack of feasible hematopoietic stem cell mobilization methods.
[0008] Chinese patent application 201310057178.5 A preparation for mobilizing hematopoietic stem cells and its separation method, using CoCl 2 and AMD3100 mobilize bone marrow mesenchymal stem cells, but there is no preparation or method for mobilizing bone marrow hematopoietic stem cells

Method used

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Experimental program
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Effect test

Embodiment 1

[0032] 1. Reagent preparation

[0033] DFO: Weigh 100mg of DFO powder, add 10ml of normal saline to make a 10mg / ml storage solution, filter it with a 0.22μm filter, and store it at -20°C. When animals were administered, the stock solution was diluted 10 times with physiological saline to adjust the concentration to 4 mg / ml. First prepare a 3mg / ml DFO solution, and then prepare a mixed solution of 2mg / ml DFO and 2mg / ml BOP.

[0034] (2) AMD3100: 5g of powder plus 5ml of normal saline to make a 1mg / ml solution, filtered through a 0.22μm filter and stored at -20°C. Physiological saline was diluted 4 times to 1.25mg / ml for administration to mice.

[0035] 2. Grouping of animals

[0036] (1) In order to detect the mobilization effect of DFO and DFO combined with AMD3100 on HSCs, the rats were divided into 6 groups with 5 rats in each group, respectively: ①Normal saline group, namely NS group: rats were given intraperitoneal injection of normal saline (with The same volume of DF...

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Abstract

The invention provides a novel preparation for mobilization of bone marrow hematopoietic stem cells. The novel preparation comprises DEO, and the dosage range of DFO is 1-15mg / kg. Preferably, DFO and BOP are combined for use. The novel preparation has the following two obvious advantages: 1, the mobilization period is short, and only one or more hours are needed for effectively collecting bone marrow stem cells from a supplier; 2, compared with a traditional method, the method has no side effect, and adverse reactions like hematological cell tumors cannot be induced.

Description

technical field [0001] In the field of biological technology, the invention specifically relates to a novel preparation for mobilizing bone marrow hematopoietic stem cells. Background technique [0002] Hematopoietic stem cells refer to immature cells, which are the origin of all blood cells and immune cells. They can not only differentiate into red blood cells, white blood cells and platelets, but also differentiate into various tissue cells across systems. Therefore, they are multifunctional stem cells. It is a "universal cell" and also the progenitor cell of the human body. [0003] Bone marrow exists in the mesh between the marrow cavity of long bones (such as humerus and femur) and the loose bone of flat bones (such as ilium). It is a spongy tissue. The bone marrow that can produce blood cells is slightly red, called red bone marrow. At birth, red bone marrow fills the bone marrow cavity of the whole body. With age, fat cells increase, and a considerable part of the r...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2500/46C12N2501/998C12N2501/999C12N2506/11
Inventor 李相鲁张严冬谢海涛
Owner GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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