Device and method for cultivating diatom

A culture device and culture method technology, applied in the field of microbiology, can solve the problems of unguaranteed quality, unstable physical and chemical factors, low biomass production, etc., and achieve the effect of saving energy consumption

Active Publication Date: 2016-08-10
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In production practice, traditional culture methods such as hanging board attachment or sand scraping and elutriation in natural sea areas are still commonly used for the cultivation of benthic diatoms. Under such cultivation conditions, various physical and chemical factors are unstable, and the biomass yield is low. The quality is not guaranteed and cannot meet the needs of production

Method used

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  • Device and method for cultivating diatom
  • Device and method for cultivating diatom
  • Device and method for cultivating diatom

Examples

Experimental program
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Effect test

Embodiment 1

[0056] In this example, two kinds of benthic diatoms were cultured, that is, algae species one: Nitzschia sp., algae species two: Amphora coffeeeformis

[0057] Algae cultivation steps: use F / 2 medium to cultivate algae; culture at 30-38°C until the concentration of algae is 0.1g / L.

[0058] F / 2 medium adds the following elements per liter of seawater: NaNO 3 75mg,NaH 2 PO4·2H 2 O5.65mg, Na 2 EDTA 4.16mg, FeCl 3 ·6H 2 O 3.15mg, Na 2 SiO 3 9H 2 O 20mg, CuSO 4 ·5H 2 O 0.01mg, ZnSO 4 ·7H 2 O 0.022mg, CoCl 2 ·6H 2 O 0.01mg, MnCl 2 4H 2 O0.18mg, Na 2 MoO 4 2H 2 O 0.006mg, Vitamin B 12 0.0005mg, Vitamin B1 0.1mg, Biotin 0.0005mg.

[0059] Large-scale cultivation steps: use a 50L translucent plastic box as a culture container, add 40L of F / 2 seawater medium into the container, pour 100mg (wet weight) of algae seeds into the medium, and put 40-80 mesh polyethylene yarn The net is fixed on the plastic frame to form a diatom attachment board for diatom attachment...

Embodiment 2

[0063] In this embodiment, culture experiments were carried out on Amphora coffeaeformis and Amphora exigua.

[0064] Algae cultivation steps: use F / 2 medium to cultivate algae; culture at 30-38°C until the concentration of algae is 0.1g / L.

[0065] Large-scale cultivation steps: Add 40 liters of F / 2 culture medium to a 50-liter culture container, the salinity of F / 2 culture medium is 35‰ to 55‰, and the algae cultured in the previous step are divided into the culture volume of the algae species and F / 2 The medium volume ratio is 1:100 and placed in the culture container, and the PC board is fixed on the plastic frame to form a diatom attachment plate for diatom attachment, and multiple diatom attachment plates are vertically placed on the attachment frame at intervals of 3 cm , blow air to the diatom attached plate, make the temperature of the seawater culture medium 35-38 ℃, irradiate the light on the diatom attached plate, the light intensity is 20μmol.m -2 .s -1 , so tha...

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Abstract

The invention discloses a device and a method for cultivating diatom. The device for cultivating the diatom comprises a cultivation container, a diatom attaching plate and inflation pipes. The cultivation container is used for accommodating cultivation media, the diatom attaching plate is arranged inside the cultivation container, and the inflation pipes are arranged inside the cultivation container and are used for blowing air to the diatom attaching plate. The method for cultivating the diatom includes a diatom seed cultivation step, a mass cultivation step, a harvesting step and a low-temperature stimulation step. According to the technical scheme, the device and the method have the advantages that problems of strict conditions, high energy consumption, vulnerability to pollution and low cell density of existing micro-diatom liquid cultivation can be solved by the aid of the device and the method; the device is simple in structure, the diatom biomass yield can be effectively increased by the device and the method, micro-diatom can be cultivated without strict conditions, the device and the method are low in energy consumption, pollution can be prevented, and high-density diatom cells can be generated.

Description

technical field [0001] The invention relates to microbiology, in particular to a diatom culture device and method. Background technique [0002] Benthic diatoms are rich in nutrients and are important bait for rare aquatic animal seedlings such as abalone, sea cucumber and sea urchin. Compared with planktonic microalgae, the cost of dehydration and harvesting of benthic diatoms is lower, but due to the attachment characteristics of benthic diatoms, the biomass production per unit of water body is much lower than that of planktonic microalgae such as Chlorella. [0003] In production practice, traditional culture methods such as hanging board attachment or sand scraping and elutriation in natural sea areas are still commonly used for the cultivation of benthic diatoms. Under such cultivation conditions, various physical and chemical factors are unstable, and the biomass yield is low. The quality is not guaranteed and cannot meet the needs of production. Contents of the inv...

Claims

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Application Information

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IPC IPC(8): C12M1/04C12M1/00C12N1/12C12N1/02C12R1/89
CPCC12M21/02C12M23/22C12M23/48C12M29/06C12M31/02C12M31/10C12N1/02C12N1/12
Inventor 刘志媛管圣马昊鑫陈宏王昕宇
Owner HAINAN UNIVERSITY
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