Method for preparing Chinese softshell turtle antihypertensive peptide by means of mixed enzymolysis and film filtration
A technology of mixed enzymatic hydrolysis and Chinese soft-shelled turtle, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of non-continuous operation, excessive degradation of substrates, low enzyme utilization rate, etc., and achieve easy industrialization Production, high conversion rate, good product quality
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Embodiment 1
[0030] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:
[0031] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, and then scald it with boiling water for 15 seconds to remove the Chinese soft-shelled turtle skin, remove the internal organs, bones, and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and after adding 5 times of water for homogenization, it is ready for use;
[0032] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to 4%, adjust the pH to 8 in a water bath at 50°C, add a mixed enzyme with 1% of the total mass of the substrate, and stir for enzymolysis After 2 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 39%, neutral protease 33%, trypsin 28%;
[0033] (3) Enzyme inactivation: at 95°C for 20 ...
Embodiment 2
[0038] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:
[0039] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, then scald it with boiling water for 20 seconds, remove the Chinese soft-shelled turtle skin, remove the internal organs, bones, and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and 10 times of water is added to homogenize it for later use;
[0040] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to 6%, adjust the pH to 9 in a water bath at 55°C, add a mixed enzyme with 2% of the total mass of the substrate, and stir for enzymolysis After 2 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 42%, neutral protease 30%, trypsin 28%;
[0041] (3) Enzyme inactivation: at 100°C for 15 minutes to inacti...
Embodiment 3
[0046] A preparation method of Chinese soft-shelled turtle antihypertensive peptide, the specific steps are as follows:
[0047] (1) Raw material pretreatment: first slaughter the Chinese soft-shelled turtle, remove the fat, then scald it with boiling water for 30 seconds, remove the Chinese soft-shelled turtle skin, remove the internal organs, bones and shells of the Chinese soft-shelled turtle and clean it; The tissue masher is used for mashing, and 3 times of water is added for homogenization and then set aside;
[0048] (2) Mixed enzymolysis: add water to adjust the mass fraction of the substrate solution in (1) to be 8%, in a water bath at 60°C, adjust the pH to 9.5, add a mixed enzyme with 3% of the total mass of the substrate, and stir for enzymolysis After 3 hours, the enzymolysis solution was obtained, wherein the mass percentages of the mixed enzyme formulas were: papain 44%, neutral protease 32%, trypsin 24%;
[0049] (3) Enzyme inactivation: at 100°C for 15 minute...
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