Trichoderma harzianum fermentation process, wettable powder and application thereof
A wettable powder, fermentation process technology, applied in the application, biocide, fungicide and other directions, can solve the problems of high cost of wettable powder, poor plant disease control effect, etc., and achieve long-lasting efficacy and good therapeutic effect. , the effect of increasing fermentation biomass
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specific Embodiment approach 1
[0021] Specific embodiment one: a kind of Trichoderma harzianum fermentation process of the present embodiment carries out according to the following steps:
[0022] 1. Selection and treatment of raw materials: choose domestic garbage or agricultural waste as raw materials, and grind them with a pulverizer after drying; Wheat bran;
[0023] Two, preparation of Trichoderma harzianum solid-state fermentation medium: add cotyledons, grape leaves, corn cobs, pineapple peels and wheat bran into the container to obtain solid-state fermentation medium for Trichoderma harzianum; said cotyledons, grape leaves, corn The mass ratio of core, pineapple peel and wheat bran is (3-6):(3-6):(3-6):(1-2):(20-41);
[0024] 3. Fermentation of Trichoderma harzianum: Get Trichoderma harzianum WY-1 bacterium liquid and add in the solid-state fermentation medium of Trichoderma harzianum prepared in step 2, the culture conditions are: initial water content 80%-100%, inoculum size 6%-10 %, 25°C-28°C f...
specific Embodiment approach 2
[0025] Embodiment 2: This embodiment is different from Embodiment 1 in that the mass ratio of the cotyledon leaves, grape leaves, corncobs, pineapple peels and wheat bran in step 2 is 4:4:4:1:27. Other steps and parameters are the same as those in the first embodiment.
specific Embodiment approach 3
[0026] Specific embodiment three: this embodiment is different from specific embodiment one in that step three culture conditions are: initial water content 100%, inoculum size 7%, 28 ° C for 10 days to complete the fermentation of Trichoderma harzianum. Other steps and parameters are the same as those in the first embodiment.
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