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Method for promoting growth of zymomonas mobilis by using philodina culture solution

A flocculating, rotifer technology, applied in biochemical equipment and methods, adding compounds to stimulate growth, bacteria, etc., can solve the problem of high L-cysteine ​​price, and achieve convenient use and flocculating bacteria. Growth-promoting, wide-ranging effects

Inactive Publication Date: 2016-07-20
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent with the publication number of patent literature CN102816797 discloses a method for enhancing the flocculation performance of the photofermentation bacterium Rhodopseudomonas faecalis by using L-cysteine, but this method has the specificity of using objects and the specificity of L-cysteine. issues such as higher prices

Method used

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  • Method for promoting growth of zymomonas mobilis by using philodina culture solution
  • Method for promoting growth of zymomonas mobilis by using philodina culture solution
  • Method for promoting growth of zymomonas mobilis by using philodina culture solution

Examples

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Embodiment 1

[0024] In this example, see figure 1 , using the spinach culture medium to promote Bacillus cereus ( Bacillus cereus ) method of growth, according to the following steps:

[0025] a. Preparation of bacterial suspension of Bacillus cereus: Inoculate the Bacillus cereus stored on the slant of the culture medium with an inoculation loop into the LB medium, then place it on a shaker at 120rpm, and cultivate it continuously at 30°C for 24 hours. Centrifuge the culture medium and wash it with deionized water. After repeated centrifugation and deionized water washing three times, adjust the bacterial concentration to OD with sterile water. 600 Value 0.6, this solution is the bacterial suspension of Bacillus cereus;

[0026] b. Preparation of simulated domestic sewage: Weigh 0.17g glucose, 0.16g starch, 0.233g sodium acetate, 0.025g ammonium chloride, 0.158g peptone, 0.04g beef extract, 0.0284g ammonium sulfate, 0.07g potassium dihydrogen phosphate, and 0.06g sodium carbonate, dis...

Embodiment 2

[0036] This embodiment is basically the same as the embodiment, and the special features are:

[0037] In this example, see figure 2 , using the culture medium of Rotorifera to promote Brevundimonas vesicularis ( Brevundimonas vesicularis ) method of growth, according to the following steps:

[0038] a. Preparation of the bacterial suspension of Brevundimonas vesicularis: inoculate the Brevundimonas vesicularis stored on the slant of the medium into the LB medium with an inoculation loop, then place it on a shaker at 120rpm, and continue at 30°C Cultivate for 24 hours, centrifuge the culture medium, wash with deionized water, repeat centrifugation and wash with deionized water three times, then adjust the bacterial concentration to OD with sterile water 600 Value 0.6, this solution is the bacterial suspension of Brevundimonas vesicularis;

[0039] b. simulated domestic sewage preparation: this step is the same as the corresponding step of embodiment one;

[0040]c. Prepa...

Embodiment 3

[0048] This embodiment is basically the same as the embodiment, and the special features are:

[0049] In this example, see image 3 , using the culture medium of Rotorifera to promote the production of Bacillus cereus ( Bacillus cereus ), Brevundimonas vesicularis ( Brevundimonas vesicularis ), Bacillus thuringiensis ( Bacillus thuringiensis ) The method for the mixed bacteria growth of these three kinds of bacteria mixed together is carried out in the following steps:

[0050] A. the preparation of the bacterium suspension of mixed bacteria: these three kinds of bacteria of Bacillus cereus, Brevundimonas vesicularis, Bacillus thuringiensis are respectively prepared into the bacterium suspension of single bacterium according to the method for step a in the embodiment one, Then the bacterial suspensions of the three bacteria are mixed in a ratio of 1:1:1 to form a mixed bacterial suspension;

[0051] b. simulated domestic sewage preparation: this step is the same as the...

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Abstract

The invention discloses a method for promoting the growth of zymomonas mobilis by using a philodina culture solution. The method comprises the following steps of firstly, culturing philodina by using a culture solution with flour as bait; when the density of the philodina reaches 100 to 500ind / mL, filtering the philodina culture solution by using a 300-mesh nylon net, and removing philodina from the philodina culture solution; secondly, filtering and separating obtained filtrate by using an ultrafiltration membrane, and further removing fine particulate matters such as flour and the like in the filtrate, thus obtaining filtrate, namely the philodina culture solution. The consumption of the philodina culture solution, disclosed by the invention, serving as a zymomonas mobilis growth promoter is 0.05 to 1 percent. The method for promoting the growth of the zymomonas mobilis by using the philodina culture solution, disclosed by the invention, has the characteristics of ecology, convenience in use and remarkable effects.

Description

technical field [0001] The invention relates to a biological treatment method for sewage and wastewater, in particular to a method for improving the flocculation of activated sludge, which is applied in the fields of microorganism cultivation and sewage and wastewater biological treatment. Background technique [0002] The activated sludge process is the most widely used method in sewage and wastewater treatment. The flocculation of activated sludge is one of the key factors affecting the operation stability, treatment efficiency and effluent quality of activated sludge treatment system. The better the flocculation of activated sludge, the better the performance of the activated sludge treatment system. The flocculation of activated sludge depends on the number and activity of flocculating bacteria. Increasing the number of flocculating bacteria or increasing the activity of flocculating bacteria are beneficial to the improvement of flocculating of activated sludge. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/38
CPCC12N1/20C12N1/38C12N2500/82
Inventor 丁国际林玮胡梦楠崔海涛涂玉娟
Owner SHANGHAI UNIV
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