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Method for extracting flavone from dendrocalamus latiflorus leaves

A technology of hemp bamboo leaves and flavonoids is applied in the field of extracting flavonoids from hemp bamboo leaves, which can solve the problems of reducing the quality of bamboo flavonoids, low purity of bamboo flavonoids, inconvenient operation, etc., so as to avoid losses, reduce production costs, and reduce difficulty. Effect

Inactive Publication Date: 2016-07-20
QIANNAN NORMAL UNIV FOR NATTIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It can be seen that in the process of continuous improvement and exploration, the extraction process of flavonoids from bamboo leaves in the prior art has more defects, such as complicated operation in the extraction process, high input cost, inconvenient operation, and the purity of bamboo flavonoids. Low, and a large number of organic solvents are used, so that a large amount of organic solvents and heavy metal ions remain in the obtained bamboo flavonoids, which reduces the quality of bamboo flavone products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for extracting flavonoids from Ma bamboo leaves, comprising crushing after low-temperature drying in the dark, leaching, standing, separating the lower layer to obtain the upper layer solution, high-speed centrifugation to remove impurities, ultrafiltration, adsorption purification, washing, eluting, Vacuum concentration and spray drying steps, wherein, in the extraction step, the pulverized Ma bamboo leaves are mixed and extracted for 6-8 hours with an ethanol aqueous solution with a volume concentration of 60-70%, and the amount of ethanol added is the pulverized Ma bamboo 16-18 times the weight of the bamboo leaves, and filter to obtain the extract; then add ammonium sulfate aqueous solution with a concentration of 0.25-0.30 g / mL to the extract, and the addition amount is 0.6-0.7 times of the extract.

Embodiment 2

[0032] On the basis of Example 1, the others are the same as in Example 1, a method for extracting flavonoids from Mazhu leaves, the extraction step is to sieve the pulverized Mazhu leaves, and then The ethanol solution with a volume fraction of 60% to 70% is used for leaching; the temperature of the ethanol solution is 70-80°C; the specific extraction process is to add 9-10 times the weight of the ethanol solution for the first time, stirring and heating to 70-80°C, control the stirring speed at 15-20r / min, stir and extract for 3-4h, filter to obtain the primary filtrate and primary filter residue; put the primary filter residue into the leaching tank with heating and stirring device, add the sieve Ethanol aqueous solution with 7-8 times the weight of the bottom material, stirred and heated to 70-80°C, the stirring speed is 15-20r / min, stirred and extracted for 3-4h, filtered to obtain the secondary filtrate and secondary filter residue; the primary filtrate and the secondary ...

Embodiment 3

[0035] A method for extracting high-purity flavonoids from the leaves of Bamboo Mamba, which comprises crushing the leaves of Bamboo Mamba, extracting them with 60% ethanol aqueous solution at 70°C for 3 hours, and filtering to obtain the extract; adding 0.60 times the extract containing The 0.25g / mL ammonium sulfate aqueous solution was mixed and stirred for 15 minutes, and the two-phase system was formed by stratification. The porous resin adsorption column is subjected to adsorption separation and purification; the macroporous resin adsorption column is washed with 50% ethanol aqueous solution as a detergent, and the macroporous resin adsorption column is analyzed with 95% ethanol aqueous solution to obtain an eluate; the eluate Concentrate into a concentrated solution under vacuum, and then spray dry the concentrated solution to obtain the powdery bamboo flavone. The said bamboo leaves should meet the following index requirements: the leaves of bamboo are normal in appeara...

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Abstract

The invention relates to the field of technologies for extracting flavone from dendrocalamus latiflorus leaves, in particular to a method for extracting flavone from dendrocalamus latiflorus leaves.The method comprises the steps that the dendrocalamus latiflorus leaves are stored and treated in a low-temperature environment after being kept out of the sun, the dendrocalamus latiflorus leaves are smashed and screened, losses of flavone are avoided, and leaching difficulty is also reduced; after ethanol water is used for leaching, filtering, two aqueous phase extraction, high-speed centrifugation treatment, ultrafiltration, macroporous resin adsorption and diluted ethanol water washing are carried out, high concentration ethanol water is adopted for decomposition, the steps of using chloroform, ethyl acetate and other organic solvents for reextraction purification are omitted, and flavone containing eluant is sprayed and dried after being concentrated in vacuum.Residues of the ethanol water are avoided, production cost is reduced, the quality of flavone is improved, the purity of the prepared flavone product is 70% or above (by rutin), no heavy metal ion is detected out, and high quality is achieved.

Description

technical field [0001] The invention relates to the technical field of extracting flavonoids from Ma bamboo leaves, in particular to a method for extracting flavonoids from Ma bamboo leaves. Background technique [0002] Dendrocalamus latiflorus Munro is a monocotyledonous plant, Poaceae, Bamboosubfamily, Dendrocalamus (Dendrocalamus) plant, also known as sweet bamboo, August hemp, widely distributed in South Asia such as Fujian, Guangdong, Guangxi, Yunnan, Guizhou and Sichuan in China Large-scale clustered bamboo species in tropical and tropical regions, especially in Sichuan and Guizhou provinces. Mazhu leaves are large, with a width of more than 4-7cm, and contain rich nutrients, which are worthy of development and utilization, such as flavonoids, phenolic acids, polysaccharides, amino acids, terpenes and volatile oils. Mazhu flavonoids are a mixture of various flavonoids, the content of which is about 1-3% in Mazhu leaves, and its main components are vitexin, rutin, lut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08C07D311/30C07D311/40
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 杨再波贺银菊毛海立向红梅
Owner QIANNAN NORMAL UNIV FOR NATTIES
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