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Biological patch for tissue damage restoration

A biological patch, tissue damage technology, applied in the field of medical devices, can solve the problems of complicated preparation, high price, etc., and achieve the effects of a good growth environment, a simple and easy method, and good tensile strength

Active Publication Date: 2016-07-20
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this natural biological patch has been commercialized, it is cumbersome to prepare and expensive

Method used

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  • Biological patch for tissue damage restoration
  • Biological patch for tissue damage restoration
  • Biological patch for tissue damage restoration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Culture and purification of Schwann cells

[0031] One-day-old SD rats were taken from newborns, killed and disinfected with alcohol, and the bilateral sciatic nerves were taken, placed on an ice-bath operating table to remove the epineurium and adhesion tissue, after the collagenase / trypsin mixed digestion was complete, the supernatant was discarded and the complete medium Resuspend the cells and inoculate them into culture dishes coated with PDL. Replace with complete medium containing cytarabine (10μM) for 24 hours and culture for 48 hours, then replace with complete medium containing HRG (50ng / ml) and Forsklin (2μM) to continue culturing, change the medium every 3 days until the cells are confluent . After the cells are fused, trypsinize and centrifuge to obtain the cell pellet, resuspend the cells with 1ml Thy1.1 complete medium (1:1000), incubate on ice for 2 hours; centrifuge to discard the supernatant, and use a mixture of DMEM and complement (3:1) Resuspe...

Embodiment 2

[0039] The preparation of embodiment 2 biological patch

[0040] (1) Preparation of elastic stamp

[0041] The elastic stamp used in the present invention is polydimethylsiloxane (polydimethylsiloxane, PDMS), and its preparation method is to mix the PDMS liquid prepared at room temperature and the cross-linking agent in a ratio of 10:1, and then cast it to the On the silicon wafer-based master template with horizontal or vertical groove-like micro-patterned distribution obtained by mask exposure, Such as figure 1 shown. Then put it into a vacuum drying oven to vacuum out air bubbles, dry and cure, and finally peel off after curing to obtain a planar PDMS elastic stamp.

[0042] (2) Preparation of micropatterned chitosan base layer

[0043] First dissolve chitosan with acetic acid to prepare a solution with a certain concentration (1%, 2% and 5%), then add the solution dropwise on PDMS, place at -60°C-90°C, freeze for 2-4 hours, and After freezing, the chitosan ice body i...

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PUM

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Abstract

The invention discloses a biological patch for tissue damage restoration. The biological patch comprises a biological material substrate layer and a cell substrate layer, wherein the substrate layer is attached onto the substrate layer; and a plurality of parallel grooves are formed in the contact surface of the substrate layer and the cell substrate layer. The substrate layer of the biological patch is simultaneously provided with porous and groove vein structures; the orientation growth and distribution of cells can be regulated and controlled; the cell substrate layer can be well attached onto the substrate layer through the mechanical property of the groove vein structures; and the falling is avoided. A product of the biological patch has the advantages that the structure is stable; the pore diameter, the porosity and the permeability are proper; the normal tissue structure can be simulated; the tissue growth pipeline and cell substrates can be provided; and cells favorable for tissue restoration and regeneration can be carried.

Description

technical field [0001] The invention relates to the field of medical devices, in particular to a biological patch for repairing tissue damage. Background technique [0002] In the real world, traffic accidents, industrial accidents, sports accidents, and clinical operations will cause tissue damage. In clinical practice, materials are often used as biological patches to replace and repair human tissues. Biological patch refers to a material taken from the same kind of tissue, which is decellularized to remove various cells contained in the tissue while retaining the three-dimensional framework structure of the extracellular matrix and can be used to repair human soft tissue. According to the tissue source, it can be divided into allogeneic materials such as dermal acellular matrix, amniotic membrane, dura mater, etc., and heterogeneous materials such as porcine small intestinal submucosa, bovine, equine pericardium, bovine peritoneum, etc. Although this natural biological p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/20A61L27/56A61L27/54A61L27/18A61L27/38A61L27/50
Inventor 杨宇民顾晓松李贵才丁斐
Owner NANTONG UNIVERSITY
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