Extracting and separating method of total flavones of wisteria flowers

A separation method and wisteria flower technology are applied in pharmaceutical formulations, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., and can solve problems such as rare reports on the extraction process of wisteria flower flavonoids, and achieve easy operation, The effect of low cost and high total flavonoid content

Inactive Publication Date: 2016-07-13
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research reports on the components of wisteria are mainly volatile oil components, and there are few reports on the extraction process of wisteria flavonoids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: Extract and isolate total flavonoids of wisteria flower according to the following steps

[0012] (1) Take fresh wisteria flowers and dry them at 70°C, crush them through a 60-mesh sieve to obtain wisteria flower powder;

[0013] (2) Put the wisteria flower powder in a heating reflux device, heat and reflux with 90% ethanol at 70°C for 2 hours, filter after heating and reflux, and repeat the reflux of the filter residue 4 times, combine the filtrate, recover the ethanol under reduced pressure, and then vacuum dry Obtain ethanol extract; wherein the mass volume ratio of wisteria powder and 90% ethanol is 1:15 (g / mL);

[0014] (3) Weigh the ethanol extract, add distilled water according to the mass of ethanol extract and distilled water volume 1:1 (g / mL); then add petroleum ether according to the mass of ethanol extract and petroleum ether volume 1:1 (g / mL) Extraction, after vigorously mixing and standing at room temperature for 24 hours to remove the petro...

Embodiment 2

[0016] Embodiment 2: Extract and isolate total flavonoids of wisteria flower according to the following steps

[0017] (1) Take fresh wisteria flowers and dry them at 70°C, crush them through a 60-mesh sieve to obtain wisteria flower powder;

[0018] (2) Put the wisteria flower powder in a heating reflux device, heat and reflux with 90% ethanol at 70°C for 2.5 hours, and filter after heating and reflux, and repeat the reflux of the filter residue 4 times, combine the filtrate, recover the ethanol under reduced pressure, and then vacuum dry Obtain ethanol extract; wherein the mass volume ratio of wisteria powder and 90% ethanol is 1:15 (g / mL);

[0019] (3) Weigh the ethanol extract, add distilled water according to the mass of ethanol extract and distilled water volume 1:1 (g / mL); then add petroleum ether according to the mass of ethanol extract and petroleum ether volume 1:1 (g / mL) Extraction, after vigorously mixing and standing at room temperature for 24 hours to remove the...

Embodiment 3

[0021] Embodiment 3: According to the following steps to extract and separate the total flavonoids of wisteria flower

[0022] (1) Take fresh wisteria flowers and dry them at 70°C, crush them through a 60-mesh sieve to obtain wisteria flower powder;

[0023] (2) Put the wisteria flower powder in a heating reflux device, heat and reflux with 90% ethanol at 70°C for 3 hours, filter after heating and reflux, and repeat the reflux of the filter residue 3 times, combine the filtrate, recover the ethanol under reduced pressure, and then vacuum dry Obtain ethanol extract; wherein the mass volume ratio of wisteria powder and 90% ethanol is 1:15 (g / mL);

[0024] (3) Weigh the ethanol extract, add distilled water according to the mass of ethanol extract and distilled water volume 1:1 (g / mL); then add petroleum ether according to the mass of ethanol extract and petroleum ether volume 1:1 (g / mL) Extraction, after vigorously mixing and standing at room temperature for 24 hours to remove t...

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PUM

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Abstract

The invention provides an extracting and separating method of total flavones of wisteria flowers.The method comprises the following steps that 1, fresh wisteria flowers are taken and dried at 70 DEG C, and the dried wisteria flowers are smashed and sieved with a 60-mesh sieve; 2, the sieved material is subjected to heating reflux at 70 DEG C with ethanol with the volume fraction of 90%, extract is dried, and the ethanol extract is obtained; 3, water is added to the ethanol extract for dispersion, the dispersed ethanol extract is extracted sequentially through petroleum ether and ethyl acetate, ethyl acetate layers are merged, solvent is removed, and an ethyl acetate extract is obtained; 4, the ethyl acetate extract is taken and separated through a D101 macroporous adsorption resin column, elution is conducted with ethanol with the volume fraction of 30% to remove impurities, ethanol with the volume fraction of 80% serves as an eluent for elution, eluant obtained in the elution process conducted with ethanol of 80% is collected, vacuum concentration and drying are conducted, and the total flavone extract of the wisteria flowers is obtained.The method is simple in technology, high in extraction rate, good in repeatability, stable and feasible.

Description

technical field [0001] The invention relates to a method for extracting and separating total flavonoids from wisteria flowers, and belongs to the field of food additives and preparation methods thereof. Background technique [0002] Wisteria flower is the inflorescence of the leguminous plant Wisteria, which is a common edible flower. Compounds have the functions of antibacterial, anti-inflammatory, improving human immunity, sedation, anti-depression, anti-oxidative free radicals, etc., and flavonoids can be used as natural food additives, antioxidants, food colorings, etc. Many health care products that use flavonoids as functional factors Food is developed. However, the current research reports on the components of wisteria are mainly volatile oil components, and there are few reports on the extraction process of wisteria flavonoids. Contents of the invention [0003] The object of the present invention is to provide a method for extracting and separating total flavono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A23L33/105
CPCA61K36/48A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 赵利琴鄢贵龙汪伟
Owner HUAIYIN TEACHERS COLLEGE
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