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Preparation method of adipose mesenchymal stem cell preparation for treating osteoarthritis

A technology of osteoarthritis and mesenchymal stem cells, applied in anti-inflammatory agents, non-central analgesics, medical preparations containing active ingredients, etc., can solve the problem of accelerating cartilage damage, weakening the therapeutic effect of osteoarthritis, cell Activity and functional impact and other issues, to achieve the effect of improving the treatment effect

Inactive Publication Date: 2016-07-13
杭州元研细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(4) Anti-inflammatory drugs mainly refer to glucocorticoids. Direct injection of this drug has a significant effect on relieving severe pain, but this drug will accelerate cartilage damage, so it cannot be used frequently
[0008] Mesenchymal stem cells have different responses to different tissue environments. When mesenchymal stem cells are in the environment of osteoarthritis, they will adjust their own cell state and secrete cytokines or related nutritional molecules suitable for improving arthritis. When mesenchymal stem cells are suddenly transplanted into the joint, they will suddenly face a strong inflammatory and free radical environment, and the cell activity and function will be affected, thus weakening the therapeutic effect of osteoarthritis

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0022] The preparation method of the adipose-derived mesenchymal stem cell preparation for the treatment of osteoarthritis described in this example, the specific steps are: 1. Preparation of arthritis animal model

[0023] (1) Prepare New Zealand white rabbits aged 6 months;

[0024] (2) Weigh 0.2g of papain, then weigh 0.2g of type II collagenase, mix the two enzymes with 10mL of normal saline, and fully dissolve them;

[0025] (3) Inject 0.3mL of mixed enzyme solution into the hip joint of New Zealand white rabbits, once every 2 days, for a total of 3 injections;

[0026] (4) After feeding under normal conditions for 2 months, New Zealand rabbits were anesthetized by injecting pentobarbital sodium, and the hip joints were opened to separate synovial tissue and cartilage tissue.

[0027] 2. Preparation of adipose-derived mesenchymal stem cells

[0028] (1) Take about 20 g of subcutaneous adipose tissue and soak it in DMEM basal medium. Under the condition of grade 100, use...

Embodiment 2

[0055] The preparation method of the adipose-derived mesenchymal stem cell preparation for the treatment of osteoarthritis described in this example, the specific steps are: 1. Preparation of arthritis animal model

[0056] (1) Prepare New Zealand white rabbits aged 10 months;

[0057] (2) Weigh 0.4g of papain, then weigh 0.2g of type II collagenase, mix the two enzymes with 10mL of normal saline, and fully dissolve them;

[0058] (3) Inject 0.5mL of mixed enzyme solution into the hip joint of New Zealand white rabbits, once every 2 days, for a total of 6 injections;

[0059] (4) After feeding under normal conditions for 2 months, New Zealand rabbits were anesthetized by injecting pentobarbital sodium, and the hip joints were opened to separate synovial tissue and cartilage tissue.

[0060] 2. Preparation of adipose-derived mesenchymal stem cells

[0061] (1) Take about 25g of subcutaneous adipose tissue and soak it in DMEM basal medium, rinse the adipose tissue with PBS und...

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Abstract

The invention relates to the field of treatment of osteoarthritis, and in particular relates to a preparation method of an adipose mesenchymal stem cell preparation for treating osteoarthritis. The method comprises: step 1, preparing microencapsulated adipose mesenchymal stem cells; step 2, preparing animal origin cartilage cells and synovial cells; step 3, performing inflammatory induction on the adipose mesenchymal stem cells; and step 4, treating the adipose mesenchymal stem cells. According to the preparation method of the adipose mesenchymal stem cell preparation for treating osteoarthritis, provided by the invention, the adipose mesenchymal stem cell preparation for treating osteoarthritis origin cells and the adipose mesenchymal stem cells are co-cultured, and a new in vitro co-culture system is invented, so that the adipose mesenchymal stem cells can obtain treatment characteristics for osteoarthritis outside a body, and can significantly promote the treatment effect on the osteoarthritis after being transplanted in to a body.

Description

technical field [0001] The invention relates to the field of osteoarthritis treatment, in particular to a method for preparing an adipose-derived mesenchymal stem cell preparation for treating osteoarthritis. Background technique [0002] Osteoarthritis is a chronic joint disease with degeneration of articular cartilage and secondary bone hyperplasia as the main symptoms. It often occurs in the knee joints, hip joints, spine and fingers with heavy loads, seriously affecting patients It is one of the most common joint diseases that affect human health. The incidence of osteoarthritis is very high, it is the fourth disease affecting women's health, and the eighth disease affecting men's health. According to statistics, the prevalence of osteoarthritis in people under the age of 45 is about 5% to 10%; but in the age of 55 to 65, the incidence rate is 40% to 60%; and in people over the age of 75, it is 80%. It is estimated that the total number of osteoarthritis patients in th...

Claims

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Application Information

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IPC IPC(8): A61K35/32A61P29/00A61P19/02A61K35/28
CPCA61K35/28A61K9/0019A61K35/32A61K47/36A61K2300/00
Inventor 徐丽君吴道安奕志娟
Owner 杭州元研细胞生物科技有限公司
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