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Amino acid modified heteropolyacid salt compound and its preparation method and application

A technology of heteropolyacid salts and compounds, applied in the field of heteropolyacid salt compounds and their preparation, capable of solving the problem of low anti-leukemia activity

Active Publication Date: 2017-12-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of low anti-leukemia activity of heteropoly compounds, and provide a heteropoly salt compound with high activity, low toxicity and low price, and an amino acid modified heteropoly salt compound

Method used

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  • Amino acid modified heteropolyacid salt compound and its preparation method and application
  • Amino acid modified heteropolyacid salt compound and its preparation method and application
  • Amino acid modified heteropolyacid salt compound and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 K 2 Na[AsMo 6 o 21 (O 2 CCH 2 NH 3 ) 3 ] · 6H 2 Synthesis of O

[0031] Na 2 MoO 4 ·H 2 O (6.0mmol), As 2 o 3 (1.1mmol), glycine (6.0mmol), KCl (6.0mmol) dissolved in H 2 O, placed on a magnetic stirrer for mixing and stirring, adjusted the pH of the solution to 3.50 with HCl, stirred and heated to reflux for 1 h. Let the solution stand until it is completely cooled, filter it into a 100ml small beaker, and let it stand for 1-2 days to form yellow crystals with a yield of 89%.

Embodiment 2

[0032] Example 2K 2Na[AsMo 6 o 21 (O 2 CCH 2 NH 3 ) 3 ] · 6H 2 Structural identification of O

[0033] (1) Compound (AsK 2 NaMo 6 o 33 C 6 h 27 N 3 ) by infrared spectroscopy detection

[0034] Using Alpha Centauri FT / IR infrared spectrometer, with KBr pellets, at 400-4000cm -1 The IR spectra of the compounds were determined in the range of . IR (KBr): 3536, 3157, 2708, 2639, 1611, 1496, 1462, 1416, 1332, 1115, 1052, 905, 778, 652, 556, 425 cm -1 。

[0035] (2) Compound (AsK 2 NaMo 6 o 33 C 6 h 27 N 3 ) UV Spectroscopic Detection

[0036] The compound was dissolved in water, and the absorption peak of the compound was measured using a UV-1800 ultraviolet-visible absorption spectrometer at a wavelength of 190-400 nm. UV spectrum see figure 1 , UV: 207nm, 226nm.

[0037] (3) compound (AsK 2 NaMo 6 o 33 C 6 h 27 N 3 ) elemental analysis

[0038] The compound (AsK 2 NaMo 6 o 33 C 6 h 27 N 3 ) for elemental analysis of C, H and N; the compoun...

Embodiment 3

[0041] Embodiment 3 MTT method measures the antitumor activity and cytotoxicity of test substance

[0042] (1) Main experimental materials

[0043] Cell lines: HL60, U937 and HUVEC cells were purchased from the Cell Bank of the Type Culture Collection Committee of the Chinese Academy of Sciences.

[0044] (2) Determination of the antitumor activity of the test substance

[0045] HL-60 and U937 cells were cultured to the logarithmic growth phase, and then 1.0×10 4 The density of cells / mL was inoculated on a 96-well plate, and different concentration gradients of the test substance (1.25, 2.5, 5, 10, 20 μM) were added, and the medium without drugs was added to the control wells, and duplicate holes were set. The same volume of culture solution was given to the negative control group. After culturing for 24, 48 and 72 h in a 5% CO2, 37°C incubator, add MTT in CO 2 After culturing in the incubator for 4 h, add 100 μL of triple solution (10% SDS, 5% isopropanol, 12 mmol / L HCl) ...

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Abstract

The invention discloses a polyoxometalate compound modified by amino acid.The compound has the general formula of Am[B3XMo6O21].nH2O and is prepared through the steps of dissolving Na2MoO4.H2O, Se, As, Sb or Bi oxide, glycine, alanine, histidine, lysine, tryptophan, phenylalanine, methionine, threonine, isoleucine, leucine or arginine and KCl in H2O, putting the solution on a magnetic stirrer to be mixed and stirred, adjusting pH of the solution to be 3.4 to 3.6 through HCl, stirring and heating reflux for 0.5-2 hours, conducting cooling and filtering, standing for 1-2 days, and collecting crystals.It is determined through the MTT method that the IC50 value of HL-60 cells is 0.127 microM, the IC50 value of U937 cells is 2.731 microM, the IC50 value of U937 cells is 2.731 microM, and the IC50 value of HUVECs cells is 889.18 microM.The polyoxometalate compound is high in leukemia resistant activity, low in toxicity and low in price.

Description

technical field [0001] The invention belongs to the field of chemical medicine, and in particular relates to an amino acid modified heteropolyacid salt compound and a preparation method and application thereof. Background technique [0002] Currently, leukemia is the sixth most lethal malignant tumor in my country. Among them, acute leukemia is a group of malignant clonal blood system diseases derived from hematopoietic stem cells and progenitor cells, which is characterized by uncontrolled cell proliferation in bone marrow and peripheral blood. The most commonly used methods for the treatment of acute leukemia include radiotherapy, chemotherapy, immunotherapy, bone marrow transplantation and other methods. Among them, the chemotherapy drugs retinoic acid and arsenic trioxide have significant effects in the treatment of acute promyelocytic leukemia, but there are still problems such as side effects and drug resistance, so finding new anti-acute leukemia drugs is a research ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C229/08C07D233/64C07C229/26C07D209/20C07C229/36C07C323/58C07C279/14C07C229/22A61P35/02
CPCC07C229/08C07C229/22C07C229/26C07C229/36C07C279/14C07C323/58C07D209/20C07D233/64
Inventor 齐燕飞李春燕李娟齐雯曹洪倩孙佳恒
Owner JILIN UNIV
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