Method for preparing lactose-free dairy product by using immobilized lactase

A technology for lactase and dairy products, applied in biochemical equipment and methods, dairy products, immobilized on or in inorganic carriers, etc., can solve the problem that lactase cannot produce lactose-free fermented milk, the enzymatic hydrolysis efficiency is reduced, and the enzyme Reduced activity and other problems, to achieve the effect of good market prospects, rich nutrition, and improved stability

Inactive Publication Date: 2016-06-29
CHANGZHOU OPTICAL MATERIAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem mainly solved by the present invention: in the process of preparing lactose-free dairy products, free lactase is often used to enzymatically hydrolyze raw milk, resulting in reduced enzyme activity or even failure in the fermentation stage, and greatly reduced enzymatic hydrolysis efficiency , even if the use of lactase is increased, the current situation of lactose-free fermented milk limited by the national standard cannot be produced. A crude enzyme solution obtained by fermentation and cultivation of Kluyveromyces fragilis is provided, which is obtained by salting out and desalting Purify lactase, cross-link it with glutaraldehyde and immobilize it on attapulgite to obtain immobilized lactase, and then use it in the enzymatic hydrolysis of raw milk to obtain lactose-free dairy products using immobilized lactase Methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0023] First, Kluyveromyces fragilis was inoculated on the potato medium according to the inoculation amount of 10%, moved into an incubator and cultured at 30°C for 3 days, scraped off the spores with a cotton swab and transferred into 200mL after sterilization, the mass concentration was 0.9% placed in a vortex shaker, oscillated and dispersed at 30W for 30 minutes to obtain a spore suspension, and filtered through a sterilized funnel to obtain spores; then press 1×10 4The amount of inoculum / mL, the spores obtained above were transferred to the fermentation medium, cultured at 25°C for 7 days and then filtered to obtain the fermentation broth, and then filtered with double-layer filter paper to remove excess spores to obtain the crude enzyme solution ; Next, measure 300 mL of the obtained crude enzyme solution, add 10 g of ammonium sulfate to it, put it in an ice-water bath at 4°C for salting out for 12 hours, and then use a high-speed centrifuge to centrifuge at a speed of 1...

example 2

[0026] First, Kluyveromyces fragilis was inoculated on potato medium at an inoculum size of 10%, moved into an incubator at 33°C for 3.5 days, scraped off the spores with a cotton swab, and transferred into 250mL of sterilized mass concentration of 0.9% placed in a vortex shaker, oscillated and dispersed at 35W for 35 minutes to obtain a spore suspension, and filtered through a sterilized funnel to obtain spores; then press 1.5×10 4 The amount of inoculum / mL, the spores obtained above were transferred to the fermentation medium, cultured at 30°C for 7.5 days and filtered to obtain the fermentation broth, and then filtered with double-layer filter paper to remove excess spores to obtain the crude enzyme solution ; Next, measure 350 mL of the obtained crude enzyme solution, add 11 g of ammonium sulfate to it, let it stand for salting out in an ice-water bath at 5°C for 13 hours, and then use a high-speed centrifuge to separate the precipitate at a speed of 11,000 r / min. After wa...

example 3

[0029] First, Kluyveromyces fragilis was inoculated on the potato medium according to the inoculation amount of 10%, moved into an incubator and cultured at 35°C for 4 days, scraped off the spores with a cotton swab and transferred into 300mL after sterilization, the mass concentration was 0.9% placed in a vortex shaker, oscillated with 40W power for 40 minutes to obtain a spore suspension, and filtered through a sterilized funnel to obtain spores; then press 2×10 4 The amount of inoculum / mL, the spores obtained above were transferred to the fermentation medium, cultured at 35°C for 8 days and then filtered to obtain the fermentation broth, and then filtered with double-layer filter paper to remove excess spores to obtain the crude enzyme solution ; Next, measure 400 mL of the obtained crude enzyme solution, add 12 g of ammonium sulfate to it, leave it to stand for salting out in an ice-water bath at 6°C for 14 hours, and then use a high-speed centrifuge to centrifuge at a spee...

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Abstract

The invention discloses a method for preparing lactose-free dairy products by using immobilized lactase, and belongs to the technical field of lactose-free dairy product preparation. In the present invention, the crude enzyme liquid is prepared by fermenting and culturing Kluyveromyces brittleness, the purified lactase is obtained through salting out and desalting, and the purified lactase is cross-linked and fixed on the attapulgite with glutaraldehyde to obtain the immobilized lactase , and then use it in the enzymatic hydrolysis of raw milk, so as to obtain a method for preparing lactose-free dairy products by using immobilized lactase. Examples prove that the present invention is easy to operate, not only improves the stability of the enzyme by using immobilized lactase, reduces the content of lactose in fermented milk, and reaches the lactose-free standard stipulated by the state, but also produces lactose-free fermented milk products with excellent taste Good, rich in nutrition, easily absorbed by the human body, suitable for large-scale production and application.

Description

technical field [0001] The invention discloses a method for preparing lactose-free dairy products by using immobilized lactase, and belongs to the technical field of lactose-free dairy product preparation. Background technique [0002] Lactose-free refers to the use of a certain process (lactase) to remove the lactose in the milk. People with too little lactase secretion should eat lactose-free milk products. [0003] Lactose is a unique disaccharide in animal milk. It cannot be directly absorbed by the human body. It must be hydrolyzed into galactose and glucose by lactase. However, because Asians do not have the habit of drinking milk on a daily basis, a large part of the population lacks lactase in their bodies. Drinking milk After the typical performance is diarrhea, that is, lactose intolerance. In addition, during the fermentation process of fermented milk, although part of the lactose is decomposed, there is still a certain amount of lactose residue. People with seve...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N9/38A23C9/12
CPCC12N11/14A23C9/1206A23C2220/104C12N9/2471C12Y302/01023
Inventor 陈毅忠张明林茂平
Owner CHANGZHOU OPTICAL MATERIAL
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