GC-MS/MS (Gas Chromatography-Mass Spectrometer/Mass Spectrometer) rapid determination method for residual amount of ametoctradin

Abstract

The invention discloses a GC-MS / MS rapid determination method for the residual amount of oxamethanil. The method is mainly used for determining the residual amount of oxamethanil in animal-derived foods with high fat content. Acetonitrile was used to homogeneously extract the residual pyraclostrobin in the sample, and the extract was dispersed and purified by Enhanced Matrix Removal (EMR) matrix, extracted and concentrated by stripping tube, and then gas chromatography-tandem mass spectrometry (GC-MS / MS) detection, using a blank matrix solution without the pesticide to be tested to establish a corrected standard working curve, and quantified by the external standard method. The average recovery rate of this method is 91.3%-99.0%, the average relative standard deviation is 5.2%-8.6%, and the detection limit is lower than 6.74 μg / kg. , Good repeatability, accurate qualitative and quantitative advantages.

Description

[0001] The invention discloses a GC-MS / MS rapid determination method for the residual amount of oxamethanil. The method is mainly used for determining the residual amount of oxamethanil in animal-derived foods with high fat content. Acetonitrile was used to homogeneously extract the residual pyraclostrobin in the sample, and the extract was dispersed and purified by Enhanced Matrix Removal (EMR) matrix, extracted and concentrated by stripping tube, and then gas chromatography-tandem mass spectrometry (GC-MS / MS) ) detection, using a blank matrix solution that does not contain the pesticide to be tested to establish a corrected standard working curve, and quantify with the external standard method. The average recovery rate of this method is 91.3%-99.0%, the average relative standard deviation (RSD) is 5.2%-8.6%, and the detection limit is lower than 6.74 μg / kg. Strong characteristics, good repeatability, and accurate qualitative and quantitative advantages. It can meet the techn...

AI Technical Summary

Problems solved by technology

[0020] At this stage, there are few studies on the determination methods of oxamethazone residues. The detection methods reported are mainly the detection methods of oxamethazone residues in vegetables and fruits. These detection methods are all based on liquid chromatography tandem mass spectrometry (LC- MS / MS) is a detection method for the determination of oxamethazone residues in vegetables and fruits. The use of LC-MS / MS to determine pesticide residues in food and agricultural products has the advantages of fast, simple, and high sensitivity. However, due to its high price, many detection methods Institutions, enterprises or scientific research institutes are not equipped with this instrument or have a small number of units. Since different compounds are detected by LC-MS / MS, different mobile phases or chromatographic columns need to be used, which requires constant replacement of chromatographic columns, mobile phases and It takes a long time to balance the system, which restricts the application of LC-MS / MS to a certain extent

The use of gas chromatography-tandem mass spectrometry (GC-MS / MS) to analyze pesticide residues in food and agricultural products has the advantages of rapidity, simplicity, high sensitivity, and strong selectivity. It can achieve multi-residue analysis of hundreds of pesticides, but so far no food and agricultural products There are reports on GC-MS / MS detection method of oxamethazone residue in food, but so far there is no report on GC-MS / MS detection method of oxamethazone residue in food and agricultural products

In addition, it is well known that the QuEChERS pretreatment technology has become the most widely used sample pretreatment method in the analysis of pesticide residues, but the QuEChERS method is mainly suitable for low-fat high-water substrates, such as most fruits and vegetables, in the face of high-fat content The capacity of the matrix is ​​limited, and it is generally necessary to add n-hexane degreasing, frozen sample extract degreasing and other steps, but the effect is limited

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