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Living organism inoculation conidium generating method for quickly obtaining panax notoginseng round spot pathogenic bacterium conidia

A technology of conidia and round spot bacteria, which is applied in the field of microorganisms, can solve the problems of long cycle, less sporulation, instability of sporulation of Acer sporogenes, etc., and achieve the effect of ensuring stability

Active Publication Date: 2016-06-22
YUNNAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0022] The purpose of the present invention is to overcome the problems such as instability, long cycle and too little sporulation of Acer sporogenes in existing methods, by studying the generation of its conidia to the demand characteristics of environmental conditions, find out A live inoculation and sporulation method for quickly obtaining conidia of Panax notoginseng, in order to stably and effectively induce more conidia in a short period of time through this method

Method used

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  • Living organism inoculation conidium generating method for quickly obtaining panax notoginseng round spot pathogenic bacterium conidia
  • Living organism inoculation conidium generating method for quickly obtaining panax notoginseng round spot pathogenic bacterium conidia
  • Living organism inoculation conidium generating method for quickly obtaining panax notoginseng round spot pathogenic bacterium conidia

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Embodiment Construction

[0033] The present invention will be described in further detail below in conjunction with the examples.

[0034] 1. Step 1—Cultivation of pathogenic bacteria: Under sterile conditions, use a puncher (Ф=5cm) to inoculate the bacteria cakes of the Acerum sp. sp. Continuous culture in medium and full light for 15 days for sporulation inoculation.

[0035] 1.1 Technical scheme design

[0036] 1.1.1 Screening of pathogenic bacteria culture temperature conditions Use a puncher (Φ=5mm) to punch out the fungus cake from the edge of the Acerella aceriformis colony cultured by the above method, move the fungus cake down to the center of the PDA plate, place Culture in dark under 10 temperature conditions of 4°C, 8°C, 12°C, 16°C, 18°C, 20°C, 22°C, 24°C, 28°C, 32°C, 11 temperature treatments in total, and each treatment received 5 Petri dish, repeated 3 times. Pure growth = average diameter of colonies - diameter of bacteria cake.

[0037] 1.1.2 Illumination condition screening set u...

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Abstract

The invention discloses a living organism inoculation conidium generating method for quickly obtaining panax notoginseng round spot pathogenic bacterium conidia. The method comprises the following steps that 1 of pathogenic bacterium culturing; 2 conidium generating device manufacturing; 3 leaf inoculating; 4 conidium inducing, wherein after inoculating is performed for about 3 days, when obviously macroscopic disease spots are formed on hypha-infected leaves, bacterium cakes are immediately removed, spray moisturizing is continuously performed, conidia are generated at the disease spots after inoculating is performed for about 6 days, the number of the conidia generated on each leaf after inoculating is performed for 10 days reaches about 104, the conidia can be washed down with sterile water in time if the conidia need to be used after the conidia are generated, 1 mL of the sterile water is sucked up with a pipettor to wash each leaf which generates the conidia, and 1 mL of conidium suspension liquid can be obtained from each leaf. Compared with the prior art, the method has the advantages that the mycocentrospora acerina conidia for scientific experiment research can be obtained in a short time, and the stability, the operability and the repeatability of an experiment can be guaranteed.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a living inoculation sporulation method for rapidly obtaining conidia of P. pseudoginseng. Background technique [0002] Mycocentrosporaacerina (R.Hartig) Deighton[=Centrosporaacerina (R.Hartig) A.G.Newhall], its taxonomic status is Ascomycota Ascomycota Dothideomycetes Mycosphaerellaceae Mycocentrospora, can parasitize in Submerged plants in freshwater basins also infect terrestrial host plants and cause plant diseases. The host range of this fungus is wide and the damage is serious. In foreign countries, it mainly causes pansy leaf spot, parsley storage period rot, carrot root rot, and burdock root rot. Diseases such as canker and crown rot, the pathogen was introduced into my country in the early 1990s, infecting the medicinal plant Asarum, causing Asarum leaf blight. According to investigation reports, Panax notoginseng plants began to be infected in 1993, resulting in the ro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 何霞红龙月娟戴蕾徐玉龙毛忠顺朱书生朱有勇
Owner YUNNAN AGRICULTURAL UNIVERSITY
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