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Germ recovery growth culture medium

A technology for recovering growth and culture medium, which is applied in the field of spore recovery growth medium, and can solve the problem of low germination rate of spores

Inactive Publication Date: 2016-06-22
NANJING JUSHA DISPLAY TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Spores germinate and grow under the induction of spore recovery growth medium, but the germination rate of spores induced by ordinary medium is extremely low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of spore recovery growth medium of bacillus stearothermophilus is made up of the raw material of following mass ratio:

[0028] Peptone 7g / L

[0029] Beef powder 4g / L

[0030] Sodium chloride 5g / L

[0031] Yeast extract 2g / L

[0032] Deionized water 1L

[0033] After stirring at room temperature until completely dissolved, use NaOH to adjust the pH to 7.2~7.4.

[0034] Sterilize at 121°30min for use.

Embodiment 2

[0036] A kind of spore recovery growth medium of bacillus stearothermophilus is made up of the raw material of following mass ratio:

[0037] Peptone 7g / L

[0038] Beef powder 4g / L

[0039] Sodium chloride 5g / L

[0040] Potassium dihydrogen phosphate 20mmol / L

[0041] Glucose 20mmol / L

[0042] Agar 20g / L

[0043] Deionized water 1L

[0044] After heating until completely dissolved, use NaOH to adjust the pH to 7.2~7.4,

[0045] Sterilize at 121°30min for use.

Embodiment 3

[0047] A kind of spore recovery growth medium of bacillus stearothermophilus is made up of the raw material of following mass ratio:

[0048] Tryptone 15g / L,

[0049] Soy peptone 3g / L,

[0050] Glucose 1.5g / L,

[0051] Dipotassium hydrogen phosphate 2g / L,

[0052] Creatinine 30mmol / L,

[0053] Sodium chloride 3.5g / L,

[0054] Agar 15g / L,

[0055] After heating until completely dissolved, use NaOH to adjust the pH to 7.2~7.4,

[0056] Sterilize at 121°20min for use.

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PUM

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Abstract

The invention provides a germ recovery growth culture medium composed of a liquid or solid matrix and a germination factor.The germination factor induces germ germination.The liquid or solid matrix provides nutritional matter or a growth environment for germs.After germ germination, the germs can be detected in subsequent experiments.The special bacillus stearothermophilus germ germination factor is added into the liquid or solid matrix, germ germination is induced, and the special bacillus stearothermophilus germ germination factor quickly induces germ germination.Meanwhile, the liquid or solid matrix provides the nutritional matter or the growth environment for the germs, and germination and recovery growth of the germs are promoted under the combined action of the germ germination factor and the liquid or solid matrix.

Description

technical field [0001] The invention relates to the field of microbial culture medium, in particular to a spore recovery growth medium of bacillus stearothermophilus. Background technique [0002] Under harsh living conditions, such as insufficient nutrients, accumulation of metabolic waste, etc., the growth of spore-forming bacteria stagnates and produces spores. Spores are dormant bodies of vegetative cells, which remain dormant for a long time and can resist a variety of external pressures, such as high temperature, high pressure, extreme cold, and nutritional deficiencies. Under appropriate conditions, usually under the activation of nutrients, it binds to the spore vegetative germination receptor, triggers intracellular cascade signal transduction, induces spore germination and restores the growth of vegetative bacteria. Spore germination is a series of irreversible and complex biochemical events that occur after microbial spores are exposed to conditions that can supp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 王卫周平乐
Owner NANJING JUSHA DISPLAY TECH
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