A method for the detection of progesterone and testosterone in saliva by high performance liquid chromatography tandem mass spectrometry
A high-performance liquid chromatography and tandem mass spectrometry technology is applied in the field of high-performance liquid chromatography-tandem mass spectrometry technology to detect progesterone and testosterone in saliva, and achieves the effects of good reproducibility, saving analysis costs and realizing batch processing.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment
[0083] 1. Instrument and parameter setting:
[0084] High performance liquid chromatography (Agilent 1100), chromatographic column (50×2.1mm, 2.6μm; Kinetex Phenomenes), mobile phase is 0.05% formic acid water and methanol, column temperature is 40°C, flow rate is 200μl·min -1 , the analysis time is 6.5min, and the liquid phase condition is gradient elution (0~1min, 20% methanol; 1~3.4min, 20%~80% methanol; 3.4~5.9min, 80% methanol; 5.9~6.1min, 80% %~20% methanol; 6.1~6.5min, 20% methanol).
[0085] A mass spectrometer (4000Qtrap, AB Company) uses an ESI source, under positive ion conditions, and the detection mode is MRM.
[0086] Detection of ion pairs (m / z): progesterone: 315.3→109.1; testosterone: 289.3→109.1; internal standard (d9-progesterone): 324.4→100.1. Curtain gas (CUR) is 10, collision gas (CAD) is 6, ion spray voltage (IS) is 5000V, temperature is 500°C, GS1 is 50, and GS2 is 50.
[0087] Mass Spectrometry Parameters for Progesterone and Testosterone and Intern...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com