Kit for detecting efficient typing of gene loci related to clopidogrel drug resistance reaction

Abstract

The invention belongs to the technical field of biology, and particularly relates to a kit for detecting efficient typing of gene loci related to clopidogrel drug resistance reaction. The kit is based on a Sequenom MassARRAY typing system, and comprises PCR (polymerase chain reaction) amplification primers and single-base extension primers of gene loci CYP2C19*2 (rs4244285) and CYP2C19*3 (rs4986893). The sequences of the PCR amplification primers of gene loci are respectively disclosed as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The sequences of the single-base extension primers of gene loci are respectively disclosed as SEQ ID NO.5 and SEQ ID NO.6. Before the subject takes clopidogrel, whether the gene loci of the subject are mutated is detected to instruct the individualized reasonable application of the subject, thereby providing references for individualized antiplatelet therapy.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for detecting and efficiently genotyping gene loci related to clopidogrel drug resistance. Background technique [0002] According to the report of the World Health Organization, cardiovascular and cerebrovascular diseases are the main cause of death worldwide. At present, there are more than 10 million patients with myocardial infarction and cerebral infarction in my country, and there are about 1 million and 2 million new patients each year. Myocardial infarction is the leading cause of death, while stroke is the leading cause of disability in adults. [0003] Antiplatelet drugs are currently one of the most important drugs for the prevention and treatment of cardiovascular and cerebrovascular diseases, and are the basic treatment in drug therapy. Clopidogrel (drug name Plavix) is currently the most commonly used antiplatelet drug in the world because of its effe...

AI Technical Summary

Problems solved by technology

In recent years, many studies in the Chinese population have found that the CYP2C19 function loss genes CYP2C19*2 (rs4244285, 681G﹥A) and CYP2C19*3 (rs4986893, 636G﹥A) will make patients undergo clopidogrel antiplatelet therapy High platelet activity state occurs when there is high platelet activity, resulting in non-response or low response to clopidogrel, and patients with loss-of-function mutations are more prone to cardiovascular and cerebrovascular ischemic events after taking clopidogrel for antiplatelet therapy

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