Hydrolysis method of capsular polysaccharide of different serotypes of Streptococcus pneumoniae

Abstract

The invention provides a hydrolysis method of different serotypes of streptococcus pneumoniae capsular polysaccharides. The hydrolysis method comprises steps as follows: a hydrogen dioxide solution and an inorganic salt solution are added to an aqueous solution of different serotypes of streptococcus pneumoniae capsular polysaccharides to hydrolyze polysaccharides, the reaction is performed for 3-5 h at the temperature of 50-80 DEG C, after the reaction is finished, a reaction mixture containing polysaccharide hydrolysis fragments with different sizes is subjected to ultrafiltration and concentration, an obtained product is purified through a Sepharose 4FF gel chromatography column, separation peaks with a kD value in a range from 0.2 to 0.65 are collected, and hydrolysis products of the different serotypes of streptococcus pneumoniae capsular polysaccharides are obtained after ultrafiltration and concentration as well as freeze-drying. With the adoption of the method, hydrolysis of the different serotypes of streptococcus pneumoniae capsular polysaccharides can be realized, the capsular polysaccharides are hydrolyzed into the fragments with different sizes, antigenicity of the hydrolysis fragments is reserved, the requirement of a preparation technology of a polyvalent pneumococcal conjugate vaccine is met, and the method can be used for preparation of the polyvalent pneumococcal conjugate vaccine applicable to infants at the age of 2 or younger.

Description

technical field [0001] The invention relates to the field of vaccine preparation, in particular to a method for hydrolyzing capsular polysaccharides of Streptococcus pneumoniae of different serotypes. Background technique [0002] Streptococcus pneumoniae or pneumococcal pneumonia is pneumonia caused by Streptococcus pneumoniae or Streptococcus pneumoniae. It usually has a sudden onset and is characterized by high fever, chills, cough, bloody sputum, and chest pain. Over the past two centuries, no infectious agent has matched the global morbidity and mortality of Streptococcus pneumoniae, responsible for the annual death of one million children under the age of 5 worldwide. Streptococcus pneumoniae remains the leading cause of community-acquired pneumonia, mainly causing lobar pneumonia in adults and bacterial pneumonia, meningitis, otitis media, and pleurisy in children. Before antibiotics, more than 70% of patients hospitalized for pneumonia bacteremia died. 20-48% of ac...

AI Technical Summary

Problems solved by technology

[0005] Due to the large molecular weight of the capsular polysaccharide of Streptococcus pneumoniae, the viscosity of the polysaccharide solution is also large, making the steps of polysaccharide activation, conjugate preparation and purification difficult to operate

The use of macromolecular polysaccharides as raw material polysaccharides will lead to excessive cross-linking of the conjugates and excessive molecular weight of the conjugates, which will pose risks to the quality of the product and may make the immune effect worse

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