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Method of separating sitosterol from extraction waste of fructus aurantii immaturus

A technology of waste and sitosterol, applied in the direction of steroids, organic chemistry, etc., can solve problems such as production limitations, and achieve high economic and environmental benefits

Inactive Publication Date: 2016-06-15
XIANGXI HUALI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many industries are extracted from some enriched waste, so the output is limited to a certain extent

Method used

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  • Method of separating sitosterol from extraction waste of fructus aurantii immaturus
  • Method of separating sitosterol from extraction waste of fructus aurantii immaturus
  • Method of separating sitosterol from extraction waste of fructus aurantii immaturus

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Take 2000ml of citrus citrus extract waste, concentrate at 100°C under normal pressure, and get a concentration of 1.08g / cm 3 Concentrate 850ml, cool the concentrate to 65°C, add petroleum ether 850ml solvent in a 65°C water bath, stir and extract for 60 minutes, let it stand for 30 minutes, separate the petroleum ether extraction layer; repeat the extraction operation with 850ml petroleum ether for the water layer 2 The second time, the petroleum ether extracts were combined and allowed to stand for 2 hours. Wet-pack the column with an appropriate amount of petroleum ether and 600 g of chromatographic silica gel. Add the extract to the silica gel column, control the speed of the column, and use the 9:1 petroleum ether: ethyl acetate mixed solution mixed solvent to elute the extract after the column is completed. The eluate is collected when sitosterol is detected by crystallization From the eluate to the eluate, no sitosterol can be detected, and a total of 8500ml of ...

Embodiment 2

[0041] Take 2000ml of citrus citrus extract waste, concentrate at 100°C under normal pressure, and get a concentration of 1.07g / cm 3Concentrate 920ml, cool the concentrate to 65°C, add petroleum ether 920ml solvent in a 65°C water bath, stir and extract for 60 minutes, let it stand for 30 minutes, separate the petroleum ether extraction layer; repeat the extraction operation with 920ml petroleum ether for the water layer 3 The second time, the petroleum ether extracts were combined and allowed to stand for 2 hours. Wet-pack the column with an appropriate amount of petroleum ether and 600 g of chromatographic silica gel. Add the extract to the silica gel column, control the speed of the column, and use the 9:1 petroleum ether: ethyl acetate mixed solution mixed solvent to elute the extract after the column is completed. The eluate is collected when sitosterol is detected by crystallization From the eluate to the eluate, no sitosterol can be detected, and a total of 9000ml of t...

Embodiment 3

[0043] Take 2000ml of citrus citrus extract waste, concentrate at 100°C under normal pressure, and get a concentration of 1.08g / cm 3 The concentrated solution is 820ml. Cool the concentrated solution to 60°C, add 820ml of cyclohexane solvent in a 65°C water bath, stir and extract for 60 minutes, let it stand for 30 minutes, and separate the cyclohexane extraction layer; the water layer is repeated with 820ml of cyclohexane The extraction was performed 3 times, and the cyclohexane extracts were combined and allowed to stand for 2 hours. Use appropriate amount of cyclohexane and 600g chromatographic silica gel to wet pack the column. Add the extract to the silica gel column, control the speed of passing through the column. After the extract is passed through the column, use 95:5 cyclohexane: ethyl acetate mixed solution mixed solvent to elute. Collect the eluate until no sitosterol can be detected in the eluate, and collect 7300ml of the eluate in total. The eluate containing ...

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Abstract

The invention relates to a method of separating sitosterol from extraction waste of fructus aurantii immaturus, wherein the method includes the steps of: extraction, column chromatography, elution, concentration, purification and drying to prepare the sitosterol. In the method, through the extraction, column chromatography and re-crystallization purification, the sitosterol in high purity is separated from the extraction waste of fructus aurantii immaturus. The purity of the sitosterol separated and extracted from the extraction waste of the fructus aurantii immaturus is higher than 98%, thereby achieving waste recycling and having high economic and environmental protective benefit.

Description

technical field [0001] The invention relates to a method for separating sitosterol from wastes extracted from Citrus aurantium. Background technique [0002] Sitosterol is a kind of substance with strong physiological activity. It has physiological activities such as lowering cholesterol, lowering blood fat, anti-tumor, and preventing heart disease. Animal growth agents and other aspects have a wide range of uses. With the sharp increase in demand in the steroid market in recent years, the demand for sterol compounds is even more in short supply, and the price is also rising year by year. Sitosterol widely exists in natural plants such as rice, wheat, corn, and rapeseed, but due to its low content, it rarely has industrial application value. Many industries are extracted from enriched waste, so the output is limited to a certain extent. [0003] At present, Citrus aurantium is mainly used in the industry to extract hesperidin, and the existing extraction methods are rough...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 王余兵饶洁石磊全铁传杨婷
Owner XIANGXI HUALI PHARMA
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