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Method for selective fluorescence recognition and colorimetric recognition of fluorine ions by using perimidine onium anion receptor

A fluorescent identification and anion technology, applied in the field of detection, can solve the problems of cumbersome, high cost, and fluoride ions cannot be used in biological assays, and achieves the effect of reducing interference errors and improving accuracy.

Inactive Publication Date: 2016-06-08
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional fluoride ion recognition methods in the prior art include electrode method, 19 FNMR nuclear magnetic resonance, etc., but these methods generally require expensive instruments and equipment and the detection process is cumbersome and the cost is high
in 19 FNMR nuclear magnetic resonance analysis is difficult to accurately detect micromolar concentration of fluoride ion
In addition, the electrode method and 19 FNMR Nuclear Magnetic Resonance Detection of Fluoride Ion Cannot be Applied in Bioassay

Method used

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  • Method for selective fluorescence recognition and colorimetric recognition of fluorine ions by using perimidine onium anion receptor
  • Method for selective fluorescence recognition and colorimetric recognition of fluorine ions by using perimidine onium anion receptor
  • Method for selective fluorescence recognition and colorimetric recognition of fluorine ions by using perimidine onium anion receptor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Fluorescence recognition of fluoride ions in DMSO solution

[0047] Pipette 1mL respectively DMSO solution of pyridinium anion acceptor (5.0×10 -5 mol / L) in a series of 10mL volumetric flasks, add 2.0mL tetrabutyl ions of fluoride ion, chloride ion, bromide ion, iodide ion, acetate ion, hydrogen sulfate ion, dihydrogen phosphate ion, perchlorate ion DMSO solution of ammonium salt (5.0×10 -4 mol / L), diluted to the mark with DMSO. At this time, the solution in The pyridinium acceptor concentration was 5.0 x 10 -6 mol / L, the concentration of anions in the solution (respectively fluoride ion, chloride ion, bromide ion, iodide ion, acetate ion, hydrogen sulfate ion, dihydrogen phosphate ion, perchlorate ion) is 1.0×10 -4 mol / L, that is, the anion concentration in each solution is 20 times that of the acceptor concentration, mix well and let stand overnight. At a concentration of 5.0×10 -6 mol / L The DMSO solution of the pyridinium anion acceptor (no anio...

Embodiment 2

[0049] Example 2: Fluorescence detection of fluoride ion content in DMSO solution

[0050] Take the concentration as 5.0×10 -6 mol / L 2mL of the DMSO solution of the pyridinium anion acceptor was gradually added to a concentration of 5.0×10 -3 mol / L fluoride ion 0-40μL, that is, the concentration of fluoride ion in the solution gradually increases from 0mol / L to 1.0×10 --4 mol / L( 20 times the concentration of pyridinium acceptor). determination Fluorescence enhancement process of pyridinium anion acceptor solution added with different concentrations of fluoride ion solution. Such as figure 2 As shown, with the increase of fluoride ion concentration, the fluorescence emission spectrum of the solution at 400nm is obviously enhanced. Take the fluorescence intensity of the solution at 400nm, and draw the relationship curve between the concentration of fluoride ions and the fluorescence intensity of the solution at 400nm, as image 3 Shown is the standard curve of fluorid...

Embodiment 3

[0053] Embodiment 3: Colorimetric identification of fluoride ions in DMSO solution

[0054] Pipette 1mL respectively DMSO solution of pyridinium anion acceptor (5.0×10 -4 mol / L) in a series of 10mL volumetric flasks, add 0.5mL tetrabutyl ions of fluoride ion, chloride ion, bromide ion, iodide ion, acetate ion, hydrogen sulfate ion, dihydrogen phosphate ion, perchlorate ion DMSO solution of ammonium salt (5.0×10 -3 mol / L), diluted to the mark with DMSO. At this time, in solution, The pyridinium anion acceptor concentration was 5.0 x 10 -5 mol / L, the concentration of anion (fluoride ion, chloride ion, bromide ion, iodide ion, acetate ion, hydrogen sulfate ion, dihydrogen phosphate ion, perchlorate ion) is 2.5×10 -4 mol / L, that is, the anion concentration in the solution is 5 times that of the acceptor concentration, mix well and place overnight. At a concentration of 5.0×10 -5 mol / L The DMSO solution of the pyridinium anion acceptor (no anion added) was used as a cont...

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Abstract

The present invention provides a method for selective fluorescence recognition and colorimetric recognition of fluorine ions by using a perimidine onium anion receptor. According to the present invention, the fluorescence emission spectrum of the perimidine onium anion receptor is significantly enhanced on the basis of the zero fluorescence after fluorine ions are added to a DMSO solution while the color of the solution is colorless from the yellow color so as to achieve the naked eye recognition; the method has advantages of real-time detection, easy operation, high sensitivity, simple detection equipment, and the like; and the perimidine onium anion receptor can achieve the effective selective recognition of the fluorine ions in the mixed solution of DMSO and water, such that the perimidine onium anion receptor shows the water phase fluorine ion recognition application prospects.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a selective fluorescence identification method and a colorimetric identification method for fluorine ions, in particular to a A method for selective fluorescence recognition and colorimetric recognition of fluoride ions by pyridinium anion receptors, and Application of pyridinium anion acceptors for fluoride ion recognition in aqueous phase. Background technique [0002] Fluoride ions widely exist in nature, and an appropriate amount of fluoride ions has a great effect on keeping teeth healthy and treating osteoporosis. However, excessive fluoride ions are difficult to excrete from the body, which can lead to serious stomach and kidney lesions. As important chemical substances, fluorine and fluorides are widely used in chemical production, medicine, military and other fields, such as the production of special plastics, toothpaste and military chemical weapons. The use of flu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/78
Inventor 高国华杨海强张大卫
Owner EAST CHINA NORMAL UNIV
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