Hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof

A magnetic nanometer and magnetic nanoparticle technology, which is applied in nanotechnology, nanomedicine, nanotechnology, etc., can solve the problems of large damage to normal tissues, large particle size of photosensitive probes, and influence on the residence time of tumor sites, so that it is not easy to enrich , Excellent magnetic properties, and long retention time at the tumor site

Active Publication Date: 2016-06-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The particle size of the photosensitive probe prepared in this patent is relatively large, which causes great damage to normal tissues and affects its residence time in tumor sites

Method used

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  • Hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof
  • Hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof
  • Hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] (1) Preparation of carboxylated magnetic particles: Weigh ferric citrate and ferrous sulfate with a molar ratio of 1:1, dissolve them in deionized water respectively, stir at a speed of 300rpm to fully dissolve them, and mix well; add a certain amount of Ascorbic acid, which reacts with Fe 3+ The molar ratio of the ions is 1:3, and the pH value is adjusted to 9 with 0.4M sodium hydroxide solution. After stirring for 30 minutes, the mixed solution is transferred to a hydrothermal reaction kettle and kept at a temperature of 150°C for 4 hours; dialysis for 1 day, Store in a refrigerator at 4°C for a long time to obtain a MNPs-COOH dispersion;

[0058] (2) PEG-modified magnetic nanoparticles: pipette 500 microliters of the MNPs-COOH dispersion, centrifuge in a 10K ultrafiltration tube, remove the supernatant, and set the parameters of the refrigerated centrifuge to: 4°C, 10,000rpm, 3min ; Then use a borate buffer solution with a pH of 7.2 to adjust the volume to the origi...

Embodiment 2

[0064] (1) Preparation of carboxylated magnetic particles: Weigh Fe 2+ and Fe 3+ Ferrous chloride and ferric citrate with a molar ratio of 1:4 were dissolved in deionized water respectively, stirred at a speed of 500rpm to fully dissolve, and mixed; add a certain amount of ascorbic acid to make it with Fe 3+ The molar ratio of the ions is 1:9, and the pH value is adjusted to 12 with 0.4M sodium hydroxide solution. After stirring for 30 minutes, the mixed solution is transferred to a hydrothermal reaction kettle, and kept at 200°C for 12 hours; dialysis for 1 day, Store in a refrigerator at 4°C for a long time to obtain a MNPs-COOH dispersion;

[0065] (2) PEG-modified magnetic nanoparticles: pipette 500 microliters of the MNPs-COOH dispersion, place it in a 10K ultrafiltration tube, and then centrifuge in a refrigerated centrifuge to remove the supernatant. The parameters of the refrigerated centrifuge are set to : 4°C, 14000rpm, 10min; then use a borate buffer solution with...

Embodiment 3

[0069] (1) Preparation of carboxylated magnetic particles: Weigh Fe 2+ and Fe 3+ Ferrous chloride and ferric acetylacetonate with a molar ratio of 1:2 were dissolved in deionized water respectively, stirred at a speed of 300rpm to fully dissolve, and mixed; add a certain amount of ascorbic acid to make it mix with Fe 3+ The molar ratio of the ions is 1:4.5, and the pH value is adjusted to 10 with 0.4M sodium hydroxide solution. After stirring for 30 minutes, the mixed solution is transferred to a hydrothermal reaction kettle and kept at 230°C for 10 hours; dialysis for 2 days, Store in a refrigerator at 4°C for a long time to obtain a MNPs-COOH dispersion;

[0070] (2) PEG-modified magnetic nanoparticles: pipette 500 microliters of the MNPs-COOH dispersion, place it in a 10K ultrafiltration tube, centrifuge in a refrigerated centrifuge, remove the supernatant, and set the parameters of the refrigerated centrifuge to : 4°C, 12000rpm, 15min; then use a borate buffer solution w...

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Abstract

The invention relates to a hyperstable monodisperse fluorescent magnetic nano probe and preparation and application thereof. The fluorescent magnetic nano probe comprises a carrier and a light-sensitive agent, wherein the carrier is composed of carboxylated ferriferrous oxide magnetic nano particles, PEG and target molecules, and the light-sensitive agent is loaded on the carrier. The preparation method includes the specific steps that firstly, ascorbic acid is placed in an ferric salt solution, the pH value of the solution is adjusted to be 9-12, stirring and hydrothermal reaction are conducted, and an MNPs-COOH dispersion solution is obtained; secondly, the MNPs-COOH dispersion solution is centrifuged, then carboxyl groups of the MNPs-COOH dispersion solution are activated, PEG is added, mixing reaction is conducted, and an MNPs-PEG dispersion solution is obtained; thirdly, activated target molecules are added into and mixed with the MNPs-PEG dispersion solution for reaction, and an MNPs-PEG dispersion solution with the target molecules is obtained; fourthly, the MNPs-PEG dispersion solution with the target molecules and the light-sensitive agent solution are stirred and mixed and react, and a target product is obtained. The nano probe is used for dual-mode tomography in animal bodies. Compared with the prior art, the hyperstable monodisperse fluorescent magnetic nano probe has the advantages that targeting efficiency is high, residence time on tumor portions is long, and the preparation technology is simple.

Description

technical field [0001] The invention relates to the field of nano-probe materials, in particular to an ultra-stable monodisperse superparamagnetic fluorescent magnetic nano-probe and its preparation and application in animal body targeting dual-mode imaging. Background technique [0002] Statistics show that the death rate of gastric cancer ranks second in the world and ranks third among malignant tumors in my country. Since gastric cancer has no designated location, it can occur in any part of the stomach. The symptoms of gastric cancer and precancerous lesions are insidious and non-specific, and the incubation period is long. Once it is discovered that it has reached an advanced stage or metastasized, the patient cannot be cured and dies . Therefore, early warning and tracing of gastric cancer cells in vivo are very important for latent patients. [0003] With the development of modern medical technology, the application of nanomaterials in medicine has entered a new per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/18A61K49/12A61K49/00A61K49/10B82Y5/00
CPCA61K49/0021A61K49/0034A61K49/0052A61K49/0054A61K49/0093A61K49/10A61K49/126A61K49/1833B82Y5/00
Inventor 高国尹婷黄鹏崔大祥
Owner SHANGHAI JIAO TONG UNIV
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