External rooting culture method of endangered relict tree species yew seed tissue culture seedlings

Abstract

The invention relates to an external rooting culture method of endangered relict tree species yew seed tissue culture seedlings. The method comprises the following steps: disinfecting surfaces of yew seeds, inoculating the disinfected yew seeds to an MS medium to make 50% of the seeds germinate 30d later, taking terminal buds of aseptic seedlings with hypocotyl with the length of 1.5cm as explants, inducing cluster buds in a medium I to make the induction rate reach 80%, cutting off new terminal buds and stem segments with lateral bud, subculturing in a medium II to obtain subcultured buds with the subculture multiplication coefficient of 7.5, repeatedly cutting off subcultured bud terminal buds and bud segments, and carrying out propagation and propagation expanding in the medium II to obtain a large amount of propagated subcultured buds; separately cutting off 4-6cm length terminal buds, carrying out rapid dipping treatment in a 500mg.L<-1> alpha-naphthylacetic acid solution, transplanting the obtained buds to an aperture disk, carrying out external rooting, and covering with a plastic aperture disk cover to carry out moisture retention; and allowing the rooting treatment to be carried out for 60d to make the rooting rate reach 64%, and transplanting rooted seedlings to a nursery, wherein the transplanting survival rate reaches 95%. The method is simple and easy, allows tissue culture rapid breeding of internal difficult-rooting tree species yews to be successfully completed, can be applied in large-scale industrial production of the endangered relict tree species yews, and solves the problem of tube asexual breeding of the yews.

Description

technical field [0001] The invention belongs to the technical field of tissue culture and propagation of endangered tree species plants, in particular to a tissue culture technology of endangered tree species yew pine, in particular to a method for cultivating roots out of a bottle of tissue cultured seedlings of yew pine seeds. Background technique [0002] Water pine Glyptostrobus pensilis Staunt.Koch, also known as water lotus, water emperor pine, metasequoia pine, water fir, water pole, long cypress, etc., water pine is deciduous or semi-deciduous tree of Taxodiaceae, which is only sporadically distributed in A single species of plants in southern provinces such as Guangdong, Guangxi, Fujian, Jiangxi, and Yunnan in my country and northern Vietnam. The water pine was born in the Mesozoic Era more than 100 million years ago. It is known as the "living fossil of plants". The water pine was widely distributed in the northern hemisphere during the Mesozoic. In the late Tertia...

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Problems solved by technology

[0004] Water pine has a high degree of dependence on the habitat, and its ability to reproduce and renew is not strong. Under natural conditions, most of the seeds fall into the water, are soaked in water, and cannot germinate; seedlings cannot grow in water for a long time because they are afraid of frost; adult trees are susceptible to Freezing damage, intolerance to dirty air, especially avoid sulfur-containing gases; cones are susceptible to insect damage, which seriously affects the seed setting rate

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