Immune colloidal gold reagent for detecting sulfamethazine and preparation method thereof

Abstract

The invention is a kind of immune colloidal gold reagent for measuring the sulfamethazole and the manufacturing method. The reagent includes a sample cushion, a colloidal gold combination cushion, aeotic acid cellulose film, a suction cushion and a PVC back lining, one end of the PVC back lining is adhered with sample cushion, colloidal gold combination cushion in order, the middle part is adhered with aeotic acid cellulose film, the other end is adhered with a suction cushion. The character lies in: the colloidal gold combination cushion is enclosed with a kind of sulfamethazole resisting specificity single clone antibody-colloidal gold label, the aeotic acid cellulose film is enclosed by sulfamethazole-BSA couplings and sheep (rabbit) antiplague IgG. The reagent has a strong specificity, high sensitivity, quick and simple, cheap, and convenient.

Description

technical field [0001] The invention relates to an immune colloidal gold reagent for detecting antibodies, and also relates to a preparation method of the reagent. Background technique [0002] Existing methods for detecting sulfamethazine mainly include high-performance liquid chromatography, gas / mass spectrometry (GC / MS), gas chromatography, thin-layer chromatography, spectrometry and wave spectrometry, capillary electrophoresis and supercritical fluid chromatography. The disadvantages of various chromatography, gas / mass spectrometry and capillary electrophoresis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technical personnel are required. Operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of...

AI Technical Summary

Problems solved by technology

The disadvantages of various chromatography, gas / mass spectrometry and capillary electrophoresis methods are: professional equipment is required; sample pretreatment procedures are cumbersome and demanding; the operation process is complicated and takes a long time; professionally trained technical personnel are required. Operators must have rich relevant experience; operators must understand various interference factors that affect chromatographic analysis, and understand the advantages and disadvantages of the pretreatment methods used in order to obtain reliable analysis results; the required equipment is expensive and difficult It is popular in cities and small and medium-sized enterprises; the requirements for instrument maintenance are high, and the quality of maintenance directly affects the accuracy of analysis results; the detection cost is high

The disadvantages of spectrometry and spectrometry are: special instruments and equipment are required, the operation process is relatively complicated, and operators also need to undergo professional training