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Lactobacillus salivarius XJP2 and application thereof

A Lactobacillus salivarius and colony technology, applied in the field of microorganisms, can solve problems such as poor tolerance, achieve good tolerance, fast in vitro culture and reproduction, and prevent bacterial diarrhea.

Inactive Publication Date: 2016-06-01
QINGDAO GENYUAN BIOLOGICAL TECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many researches and applications of lactic acid bacteria, and there are many types of related products in the market, but the common problem is that lactic acid bacteria have poor tolerance to acid and bile salts, can they be planted in the intestinal mucosa, survive and play a role in the intestinal tract for a long time

Method used

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  • Lactobacillus salivarius XJP2 and application thereof
  • Lactobacillus salivarius XJP2 and application thereof
  • Lactobacillus salivarius XJP2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, the screening of Lactobacillus salivarius

[0019] 1. Isolation and screening of Lactobacillus salivarius XJP2

[0020] Taking healthy broiler chickens as samples, scrape the cecal mucosa with sterile glass slides and put it in sterile water, shake and mix well for gradient dilution, pour the MRS medium into the culture and screen the colonies with obvious "calcium dissolution circle", and obtain a single colony after multiple purifications , name it XJP2, and save it. The MRS medium is a general medium for cultivating lactic acid bacteria, and its formula is: peptone 10g / L, beef extract 10g / L, yeast powder 5g / L, glucose 20g / L, Tween 801ml / L, dipotassium hydrogen phosphate 2g / L, sodium acetate 5g / L, triammonium citrate 2g / L, magnesium sulfate 0.58g / L, manganese sulfate 0.25g / L, calcium carbonate 5g / L, pH6.2.

[0021] The colony morphology of described bacterial strain XJP2 on MRS plate is as follows figure 1 As shown, its biological characteristics: Gr...

Embodiment 2

[0025] Embodiment 2, the growth assay of Lactobacillus salivarius XJP2

[0026] The activated XJP2 bacterial solution was mixed with 1% (about 10 7 cfu / mL) was inoculated in the MRS liquid medium, cultured at 37° C. and 160 rpm for 24 h, sampling every 3 h, measuring the absorbance at 600 nm, and drawing a growth curve. Such as figure 2 As shown, the experimental results show that XJP2 enters the logarithmic growth phase after 3 hours of culture, and enters the stationary phase after 12 hours. The output of L-lactic acid was 18.2g / L when cultured for 20h.

Embodiment 3

[0027] Embodiment 3, Lactobacillus salivarius XJP2 is to the tolerance determination of acid, bile salt

[0028] The activated XJP2 bacterial solution was mixed with 1% (about 10 7 cfu / mL) were inoculated in MRS medium with pH of 2.5, 3.5, and 4.5, respectively, and cultured statically at 37°C. Samples were taken at 0h, 2h, and 4h, and MRS was poured and counted. Treat the bacterial strain in the same way, inoculate the bacterial liquid in the MRS medium with pig bile salt concentration of 0.1%, 0.2%, and 0.3%, and culture it statically at 37°C. Samples were taken at 0h, 2h, and 4h, and MRS was poured and counted.

[0029] From the results in Table 1 and Table 2, it can be seen that the strain XJP2 has good tolerance to acid and bile salts, and the survival rate of viable bacteria exceeds 70% after being cultured in MRS medium with pH 2.5 or 0.3% bile salts for 2 hours , has better stress resistance.

[0030] Table 1 The results of acid tolerance of strain XJP2 (cfu / mL)

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Abstract

The invention provides lactobacillus salivarius XJP2 and application thereof. The lactobacillus salivarius XJP2 is separated from the cecum mucosa of a healthy broiler chicken and preserved in the China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.11386. The lactobacillus salivarius XJP2 has the better tolerance to acids and cholate and can be colonized in the animal intestinal tract. The lactobacillus salivarius XJP2 is high in in-vitro culture reproduction speed and L-lactic acid yield, has the functions of preventing bacterial diarrhea of the broiler chickens, improving the intestinal flora and the like and can be applied to development of microecological preparations.

Description

technical field [0001] The invention mainly relates to the technical field of microorganisms, in particular to a strain of Lactobacillus salivarius XJP2 and its application. Background technique [0002] Under normal circumstances, the intestinal flora maintains a balanced relationship with the animal body to maintain the health of the body: on the one hand, the pathogenic bacteria entering the body compete for the mucosal attachment sites through the bacterial film barrier or competitive exclusion, so that the pathogenic bacteria cannot colonize and strengthen the body. Immunity, reduce the incidence of disease; on the other hand, the flora participates in the body's material metabolism, nutrient transformation and synthesis, etc., and promotes the healthy growth of animals. Lactic acid bacteria are one of the beneficial flora in animals. They are the normal colonization flora in the intestinal tract of animals. They have multiple functions such as biological antagonism, im...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/75C12R1/225
CPCC12N1/205C12R2001/225
Inventor 王娇王欣志于刚赵世伟张大伟赵志强王政
Owner QINGDAO GENYUAN BIOLOGICAL TECH GRP
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